Main Article Content

Abstract

Cajuput (Melaleuca spp.) is a high-value non-timber forest product in Seram Island, Maluku. The quality of cajuput oil is primarily determined by its 1,8-cineole (eucalyptol) content, which is influenced by genetic, environmental, and raw material factors. This study analyzed the effects of species, growing location, and raw material condition on the yield and chemical composition of oil derived from Melaleuca leucadendra and Melaleuca cajuputi collected from Hatusua and Eti villages, using distillation and GC-MS analysis. The results showed that fresh leaves yielded higher amounts than dried leaves. The highest yield was obtained from M. leucadendra from Eti (1.022%), while the lowest yield was recorded from dried leaves (0.008%). M. leucadendra oil was dominated by eucalyptol (56.62–58.50%), whereas M. cajuputi was dominated by methyleugenol (81.61%) with a lower yield (0.1213%), reflecting differences in genetically controlled metabolic pathways. Environmental factors influenced yield and minor compounds without altering the dominant constituents. Overall, the quality and quantity of cajuput oil are determined by the interactions among genetic, environmental, and post-harvest factors. The use of fresh raw materials, appropriate species or chemotypes, and optimal growing locations is key to improving oil quality and ensuring consistent production.   

Keywords

Cajuput oil, melaleuca leucadendra, melaleuca cajuputi, eucalyptol, GC-MS

Article Details

How to Cite
1.
Kapelle IBD, Fensia Analda Souhoka, Nini Munirah Renur. Influence of Species, Growing Site, and Postharvest Leaf Handling on the Yield and Chemical Composition of Cajuput Oil (Melaleuca spp.) from Seram Island, Indonesia. EKSAKTA [Internet]. 2026 Jun. 3 [cited 2026 Jun. 4];27(03):275-84. Available from: https://eksakta.ppj.unp.ac.id/index.php/eksakta/article/view/687

References

  1. [1] Kapelle, I. B. D., Souhoka, F. A., Rosmawaty, Jani, A. U. B., Jelita, W. P., & Silahooy, V. B. (2025). Chemical composition of essential oils reviewed from the height of Cajuput (Melaleuca leucadendron) plantations in Buru Island and Seram Island, Maluku, Indonesia. Open Chemistry, 23(1), 20250172.
  2. [2] Raza, M. Q., Sufyan, M., Riaz, A., Lail, N. U., Fatima, S. R., Shoukat, F., ... & Talib, A. (2024). Impressive Benefits Of Eucalyptus Leaves. Kashf Journal of Multidisciplinary Research, 1(10), 25-39.
  3. [3] Shiekh, R. A. E., Atwa, A. M., Elgindy, A. M., Mustafa, A. M., Senna, M. M., Alkabbani, M. A., & Ibrahim, K. M. (2025). Therapeutic applications of eucalyptus essential oils: RA El Shiekh et al. Inflammopharmacology, 33(1), 163-182.
  4. [4] Salvatori, E. S., Morgan, L. V., Ferrarini, S., Zilli, G. A., Rosina, A., Almeida, M. O., ... & Dal Magro, J. (2023). Anti‐Inflammatory and Antimicrobial Effects of Eucalyptus Spp. Essential Oils: A Potential Valuable Use for an Industry Byproduct. Evidence‐Based Complementary and Alternative Medicine, 2023(1), 2582698.
  5. [5] Moussa, H. H., Sara, B., Benhalima, H., Benaliouche, F., Sbartai, I., & Sbartai, H. (2024). Chemical characterization of Eucalyptus (Eucalyptus globulus) leaf essential oil and evaluation of its antifungal, antibacterial and antioxidant activities. Cellular and Molecular Biology, 70(12), 1-9.
  6. [6] Mieres-Castro, D., Ahmar, S., Shabbir, R., & Mora-Poblete, F. (2021). Antiviral activities of eucalyptus essential oils: their effectiveness as therapeutic targets against human viruses. Pharmaceuticals, 14(12), 1210.
  7. [7] Shala, A. Y., & Gururani, M. A. (2021). Phytochemical properties and diverse beneficial roles of Eucalyptus globulus Labill.: A review. Horticulturae, 7(11), 450.
  8. [8] Yoro, T., Alioune, D., Abdoulaye, D., Jean, C., Saad Bouh, C. B., Alassane, W., & Julien, P. (2020). Essential oil of Eucalyptus alba L. Growing on the Salt Zone of Fatick (Senegal) as a Source of 1, 8-Cineole and Their Antibacterial Activity. J. Drug Deliv. Ther, 10, 140-143.
  9. [9] Tine, Y., Diallo, A., Ndoye, I., Gaye, C., Ndiaye, B., Diop, A., ... & Paolini, J. (2022). Chemical Variability and Antibacterial Activity of Eucalyptus camaldulensis Essential Oils from Senegal. International Journal of Organic Chemistry12, 173-180.
  10. [10] Vázquez, A., Tabanca, N., & Kendra, P. E. (2023). HPTLC analysis and chemical composition of selected Melaleuca essential oils. Molecules, 28(9), 3925.
  11. [11] Borotová, P., Galovičová, L., Vukovic, N. L., Vukic, M., Tvrdá, E., & Kačániová, M. (2022). Chemical and biological characterization of Melaleuca alternifolia essential oil. Plants, 11(4), 558.
  12. [12] Fikry, E., Orfali, R., Perveen, S., Ghaffar, S., El-Shafae, A. M., El-Domiaty, M. M., & Tawfeek, N. (2025). Chemical Composition and Anti-Lung Cancer Activities of Melaleuca quinquenervia Leaf Essential Oil: Integrating Gas Chromatography–Mass Spectrometry (GC/MS) Profiling, Network Pharmacology, and Molecular Docking. Pharmaceuticals, 18(6), 771.
  13. [13] Tran, P. H., Vu, T. T. T., Phan, T. D. T., Nguyen, V. M., Ngo, T. N. M., Le, C. V. C., & Ton, T. H. D. (2024). Chemical compositions and biological properties of the leaf essential oil of three Melaleuca species. World Academy of Sciences Journal, 6(6), 67.
  14. [14] Arisandi, R., Pujiarti, R., Lukmandaru, G., & Mulyana, B. (2023). Chemical constituents of Melaleuca leucadendron Linn. leaf essential oils quality under different collecting time in KPH Yogyakarta, Gunungkidul, Indonesia. Indonesian Journal of Forestry Research, 10(2), 195-205.
  15. [15] Pant, P., Pandey, S., & Dall'Acqua, S. (2021). The influence of environmental conditions on secondary metabolites in medicinal plants: A literature review. Chemistry & biodiversity, 18(11), e2100345.
  16. [16] Abdelmohsen, U. R., & Elmaidomy, A. H. (2025). Exploring the therapeutic potential of essential oils: a review of composition and influencing factors. Frontiers in Natural Products, 4, 1490511.
  17. [17] Kholiya, S., Bhatt, G., Chauhan, A., Kumar, D., KT, V., Upadhyay, R. K., & Padalia, R. C. (2023). Effect of seasons, storage and distillation times on essential oil composition of Melaleuca leucadendra (L.). Indian Journal of Natural Products and Resources (IJNPR)[Formerly Natural Product Radiance (NPR)], 14(4), 611-616.
  18. [18] Wang, P., Chen, X., Wei, X., Xiong, B., Pan, X., Bai, J., ... & Xu, X. (2025). Effects of different drying methods on physical properties and anthocyanin and volatile compound contents of black sweet corn (Zea mays L. Saccharata Sturt). Frontiers in Nutrition, 12, 1682022.
  19. [19] Qin, H. W., Yang, T. M., Yang, S. B., Yang, M. Q., Wang, Y. Z., & Zhang, J. Y. (2022). Effects of different pre-drying and drying methods on volatile compounds in the pericarp and kernel of Amomum tsao-ko. Frontiers in Plant Science, 13, 803776.
  20. [20] Osik, N. A., Lukzen, N. N., Yanshole, V. V., & Tsentalovich, Y. P. (2024). Loss of volatile metabolites during concentration of metabolomic extracts. ACS omega, 9(22), 24015-24024.
  21. [21] Calín-Sánchez, Á., Lipan, L., Cano-Lamadrid, M., Kharaghani, A., Masztalerz, K., Carbonell-Barrachina, Á. A., & Figiel, A. (2020). Comparison of traditional and novel drying techniques and its effect on quality of fruits, vegetables and aromatic herbs. Foods, 9(9), 1261.
  22. [22] Mujumdar, A. S., & Menon, A. S. (2020). Drying of solids: principles, classification, and selection of dryers. In Handbook of industrial drying (pp. 1-39). CRC Press.
  23. [23] Jan, R., Asaf, S., Numan, M., Lubna, & Kim, K. M. (2021). Plant secondary metabolite biosynthesis and transcriptional regulation in response to biotic and abiotic stress conditions. Agronomy, 11(5), 968.
  24. [24] Laftouhi, A., Eloutassi, N., Ech-Chihbi, E., Rais, Z., Abdellaoui, A., Taleb, A., ... & Taleb, M. (2023). The impact of environmental stress on the secondary metabolites and the chemical compositions of the essential oils from some medicinal plants used as food supplements. Sustainability, 15(10), 7842.
  25. [25] Alfalah, M., Bouharroud, R., Beniaich, A., El Aroussi, F., El Gharous, M., & Lyamlouli, K. (2025). Phosphorus-drought interaction modulates growth dynamics and essential oil biosynthesis in Rosmarinus officinalis. Frontiers in Plant Science, 16, 1646658.
  26. [26] Azimzadeh, Z., Hassani, A., Mandoulakani, B. A., Sepehr, E., & Morshedloo, M. R. (2023). Intraspecific divergence in essential oil content, composition and genes expression patterns of monoterpene synthesis in Origanum vulgare subsp. vulgare and subsp. gracile under salinity stress. BMC plant biology, 23(1), 380.
  27. [27] Mansinhos, I., Gonçalves, S., & Romano, A. (2024). How climate change-related abiotic factors affect the production of industrial valuable compounds in Lamiaceae plant species: a review. Frontiers in Plant Science, 15, 1370810.
  28. [28] Ninčević Runjić, T., Pljevljakušić, D., Runjić, M., Grdiša, M., & Šatović, Z. (2025). Phenotypic plasticity vs. local genetic adaptation: essential oil diversity of natural immortelle (Helichrysum italicum (Roth.) G. Don) populations along eastern Adriatic coast. Frontiers in plant science, 16, 1467421.
  29. [29] Pluhár, Z., Kun, R., Cservenka, J., Neumayer, É., Tavaszi-Sárosi, S., Radácsi, P., & Gosztola, B. (2024). Variations in essential oil composition and chemotype patterns of wild thyme (Thymus) species in the natural habitats of Hungary. Horticulturae, 10(2), 150.
  30. [30] Mulugeta, S. M., Pluhár, Z., & Radácsi, P. (2023). Phenotypic variations and bioactive constituents among selected Ocimum species. Plants, 13(1), 64.
  31. [31] Warren-Walker, A., Beckmann, M., Watson, A., McAllister, S., & Lloyd, A. J. (2025). Effect of thermal processing by spray drying on key ginger compounds. Metabolites, 15(6), 350.
  32. [32] Deng, J., Hou, M., Cui, S., Liu, Y., Li, X., & Liu, L. (2025). Integrative analysis of transcriptome and metabolome reveals molecular mechanisms of dynamic change of storage substances during dehydration and drying process in peanuts (Arachis hypogaea L.). Frontiers in Plant Science, 16, 1567059.
  33. [33] Xing, J., Yang, L., Zhang, L., Han, J., & Cai, E. (2025). Widely targeted metabolomics analyses provide insights into the transformation of active ingredients during drying and the mechanisms of color change for forest ginseng (Panax ginseng CA mey. Cv. Sativi-nemoralis). Plants, 14(3), 494.
  34. [34] Zhang, T., Zheng, Y., Fu, C., Yang, H., Liu, X., Qiu, F., ... & Wang, Z. (2023). Chemical variation and environmental influence on essential oil of Cinnamomum camphora. Molecules, 28(3), 973.
  35. [35] Cruz, E. D. N. S. D., Barros, L. D. S. P., Guimarães, B. D. A., Mourão, R. H. V., Maia, J. G. S., Setzer, W. N., ... & Figueiredo, P. L. B. (2023). Seasonal variation in essential oil composition and antioxidant capacity of Aniba canelilla (Lauraceae): a reliable source of 1-Nitro-2-phenylethane. Molecules, 28(22), 7573.
  36. [36] Rezaie, R., Abdollahi Mandoulakani, B., & Fattahi, M. (2020). Cold stress changes antioxidant defense system, phenylpropanoid contents and expression of genes involved in their biosynthesis in Ocimum basilicum L. Scientific reports, 10(1), 5290.
  37. [37] Yang, C., Lin, Y., Xiang, X., Shao, D., Qiu, Z., Li, Y., & Wu, S. (2024). MbEOMT1 regulates methyleugenol biosynthesis in Melaleuca bracteata F. Muell. Tree Physiology, 44(4), tpae034.
  38. [38] Lv, M., Zhang, L., Wang, Y., Ma, L., Yang, Y., Zhou, X., ... & Li, S. (2024). Floral volatile benzenoids/phenylpropanoids: biosynthetic pathway, regulation and ecological value. Horticulture Research, 11(10), uhae220.
  39. [39] Kuspradini, H., Kartiko, A. B. B., Putri, A. S., Suwinarti, W., & Rosamah, E. (2025). Exploring the essential oil from wild Melaleuca leucadendra with trans-caryophyllene as the predominant compound for its antioxidant properties.