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Submitted
Mar 15, 2025
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Apr 21, 2025
Published
May 2, 2025
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Abstract

The bark of Cinnamomum culilaban, endemic to Southeast Maluku, has the potential as an antibacterial. This study aimed to determine the antibacterial activity of C. culilaban bark extract isolated using distillation and maceration methods and to determine the chemical components contained therein. Qualitative tests using GCMS and antibacterial activity testing against E. coli and S. aureus bacteria. The extraction results using the distillation method obtained a sedimentation of 3.28% and the maceration method of 28.03%. The chemical components of the distillation extract showed four main components: Eucalyptol 4.02%, (+)-2-Bornanon 2.32%, terpineol 1.49% and Safrole 86.78%. The chemical components of the maceration extract have five main components, namely (+)-2-Bornanon 1.35%, Terpineol 1.43%, Safrole 89.06%, Spathulenol 1.26%, and Methoxyeugenol 1.73%. The antibacterial activity of the distillation extract against E. coli bacteria is classified as strong, with the highest inhibition zone size at a concentration of 60% (13.47 ± 1.14 mm). In contrast, for S. aureus bacteria, there is no antibacterial activity. The antibacterial activity of the maceration extract against S. aureus bacteria is classified as strong with the inhibition zone size at a concentration of 100% (11.87 ± 1.15 mm). In contrast, for E. coli bacteria, there is no antibacterial activity.

Keywords

Cinnamomum culilaban (L.) Presl, Lawang bark extract, antibacterial, GCMS analysis

Article Details

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite
1.
Kapelle IBD, Shielda Natalia Joris, Fauzan Saleh, Nini Munirah Renur. Chemical Components and Antibacterial Activity of Cinnamomum culilaban Extract from Southeast Maluku. EKSAKTA [Internet]. 2025May2 [cited 2025May8];26(02):163-74. Available from: https://eksakta.ppj.unp.ac.id/index.php/eksakta/article/view/584

References

  1. Larsson, D.G.J., & Flach, C.F. (2022). Antibiotic resistance in the environment. Nat Rev Microbiol, (20), 257–269.
  2. Tang, K.W.K., Millar, B.C., & Moore, J.E. (2023). Antimicrobial Resistance (AMR). Br J Biomed Sci, (80), 11387.
  3. Taylor, T.A., & Unakal, C.G. (2025). Staphylococcus aureus Infection. [Updated 2023 Jul 17]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing. Available from: https://www.ncbi.nlm.nih.gov/books/NBK441868/
  4. Mansour, N.A, Loubet, P., Pouget, C., Dunyach-Remy, C., Sotto, A., Lavigne, J.P., & Molle, V. (2021). Staphylococcus aureus Toxins: An Update on Their Pathogenic Properties and Potential Treatments. Toxins (Basel), 13(10), 677.
  5. Mueller, M., & Tainter, C.R. (2023). Escherichia coli Infection. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing. Available from: https://www-ncbi-nlm-nih-gov.translate.goog/books/NBK564298/?_x_tr_sl=en&_x_tr_tl=id&_x_tr_hl=id&_x_tr_pto=tc.
  6. Salam, M.A., Al-Amin, M.Y., Salam, M.T., Pawar, J.S., Akhter, N., Rabaan, A.A., & Alqumber, M.A.A. (2023). Antimicrobial Resistance: A Growing Serious Threat for Global Public Health. Healthcare (Basel), 11(13), 1946.
  7. Wibowo, M.A., Sari, D,N,, Jayuska, A., & Ardiningsih, P. (2021). Komposisi Kimia Dan Uji Aktivitas Antibakteri Minyak Atsiri Daun Kayu Putih (melaleuca Cajuputi) Dari Kota Singkawang. Biopropal Industri, 12(1), 1-7.
  8. Chukwuma, I.F., Uchendu, N.O., Asomadu, R.O., Ezeorba, W.F.C., & Ezeorba, T.P.C. (2023). African and Holy Basil - a review of ethnobotany, phytochemistry, and toxicity of their essential oil: Current trends and prospects for antimicrobial/anti-parasitic pharmacology. Arabian Journal of Chemistry, 16(7), 104870.
  9. Saleh, Z.M., Azeiz, A.Z., Mehany, A.B.M., & El-Swaify, Z.A.S. (2023). Anticancer and Antimicrobial Activity of Jatropha’s Leaves Extracts. Egyptian Journal of Botany, 63(2), 621-634.
  10. Vaou, N., Stavropoulou, E., Voidarou, C.C., Tsakris, Z., Rozos, G., Tsigalou, C., & Bezirtzoglou, E. (2022). Interactions between Medical Plant-Derived Bioactive Compounds: Focus on Antimicrobial Combination Effects. Antibiotics (Basel), 11(8), 1014.
  11. Siddiqui, S.A., Khan, S., Mehdizadeh, M., Bahmid, N.A., Adli, D.N., Walker, T.R., Perestrelo, R., & Câmara, J.S. (2023). Phytochemicals and bioactive constituents in food packaging. A systematic review, 9(11), e21196.
  12. George, A.S., & Brandl, M.T. (2021). Plant Bioactive Compounds as an Intrinsic and Sustainable Tool to Enhance the Microbial Safety of Crops. Microorganisms, 9(12), 2485.
  13. Intan, K., Diani, A., & Nurul, A.S.R. (2021). Aktivitas Antibakteri Kayu Manis (Cinnamomum burmanii) terhadap Pertumbuhan Staphylococcus aureus. Jurnal Kesehatan Perintis, 8(2), 121-127.
  14. Saki, M., Seyed-Mohammadi, S., Montazeri, E.A., Siahpoosh, A., Moosavian, M., & Latifi, S.M. (2020). In vitro antibacterial properties of Cinnamomum zeylanicum essential oil against clinical extensively drug-resistant bacteria. European Journal of Integrative Medicine, (37), 101146.
  15. Twaij, B.M., & Hasan, M.N. (2022). Bioactive Secondary Metabolites from Plant Sources: Types, Synthesis, and Their Therapeutic Uses. International Journal of Plant Biology, 13(1), 4-14.
  16. Ku, Y.S., Contador, C.A., Ng, M,S., Yu, J., Chung, G., & Lam, H.M. (2020). The Effects of Domestication on Secondary Metabolite Composition in Legumes. Front Genet, (11), 581357.
  17. Mungwari, C.P., King'ondu, C.K., Sigauke, P., Obadele, B.A. (2025). Conventional and modern techniques for bioactive compounds recovery from plants: Review. Scientific African, (27), e02509.
  18. Bitwell, C., Indra, S.S., Luke, C., & Kakoma, M.K. (2023). A review of modern and conventional extraction techniques and their applications for extracting phytochemicals from plants. Scientific African, (19), e01585.
  19. Das, G., Patra, J.K., Kang, S.S., & Shin, H.S. Pharmaceutical Importance of Some Promising Plant Species with Special Reference to the Isolation and Extraction of Bioactive Compounds: A Review. Curr Pharm Biotechnol, 23(1), 15-29.
  20. Kurniatri, A.A., Elya, B., & Suryadi, B. (2024). Antibacterial Potential of Cinnamomum culilaban Bark Ethanolic Extract Prepared by Ultrasound-Assisted Extraction against Oral Pathogens. Jurnal Kefarmasian Indonesia, 14(2), 204-211.
  21. Fitriansyah, S.N., Ruslan, K., Rizky, D.N., Munthary, D., & Riasari, H. (2024). Optimization of Andrografolid Extraction from the Herb Sambiloto (Andrographis Paniculata (Burm.F.) Nees) Using a Combined Maceration and Refluction Method. Trop J Nat Prod Res, 8(12), 9529 – 9536.
  22. Prem, N., Satyal, P., & Setzer, W. (2023). Chemical-Enantiomeric Characterization and In-Vitro Biological Evaluation of the Essential Oils from Elsholtzia strobilifera (Benth.) Benth. and E. blanda (Benth.) Benth. from Nepal. Natural Product Communications, (18), 1-11.
  23. Bayot, M.L., & Bragg, B.N. (2025). Antimicrobial Susceptibility Testing. [Updated 2024 May 27]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing, Available from: https://www.ncbi.nlm.nih.gov/books/NBK539714/
  24. Hossain, T.J. (2024). Methods for screening and evaluation of antimicrobial activity: A review of protocols, advantages, and limitations. Eur J Microbiol Immunol (Bp), 14(2), 97-115.
  25. Zhang, L., Zhang, M., Ju, R., Bhandari, B., & Liu, K. (2023). Antibacterial mechanisms of star anise essential oil microcapsules encapsulated by rice protein-depolymerized pectin electrostatic complexation and its application in crab meatballs. International Journal of Food Microbiology, (384), 109963.
  26. Galgano, M., Capozza, P., Pellegrini, F., Cordisco, M., Sposato, A., Sblano, S., Camero, M., Lanave, G., Fracchiolla, G., Corrente, M., Cirone, F., Trotta, A., Tempesta, M., Buonavoglia, D., & Pratelli, A. (2022). Antimicrobial Activity of Essential Oils Evaluated In Vitro against Escherichia coli and Staphylococcus aureus. Antibiotics (Basel), 11(7), 979.
  27. Gorman, A., & Golovanov, A.P. (2022). Lipopolysaccharide Structure and the Phenomenon of Low Endotoxin Recovery. European Journal of Pharmaceutics and Biopharmaceutics, (180), 289-307.
  28. Gallardo, M.G., Eckhard, U., Delgado, L.M., de Roo Puente, Y.J.D., Nogués, M.H., Gil, F.J., & Perez, R.A. (2021). Antibacterial approaches in tissue engineering using metal ions and nanoparticles: From mechanisms to applications. Bioactive Materials, 6(12), 4470-4490.
  29. Martínez, F.J.A., Catalán, E.B., López, M.H., & Micol, V. (2021). Antibacterial plant compounds, extracts and essential oils: An updated review on their effects and putative mechanisms of action. Phytomedicine, (90), 153626.
  30. Uddin, T.M., Chakraborty, A.J, Khusro, A., Zidan, B.M.R.M., Mitra, S, Emran, T.B., Dhama, K., Ripon M.K.H., Gajdács, M., Sahibzada, M.U.K., Hossain, M.J., & Koirala, N. (2021). Antibiotic resistance in microbes: History, mechanisms, therapeutic strategies and future prospects. Journal of Infection and Public Health, 14(12), 1750-1766.
  31. Anggraeni, N.D., Nurjanah, S., & Lembong, E. 2020. Antibacterial Activity of -guaiene Patchouli Oil on Staphylococcus aureus and Staphylococcus epidermidis. Gontor AGROTECH Science Journal, 6(3).
  32. Cunliffe, A.J., Askew, P.D., Stephan, I., Iredale, G., Cosemans, P., Simmons, L.M., Verran, J., & Redfern, J. (2021). How Do We Determine the Efficacy of an Antibacterial Surface? A Review of Standardised Antibacterial Material Testing Methods. Antibiotics (Basel), 10(9), 1069.
  33. Alamsjah, F., Fandini, S., & Mildawati, M. (2024). Short Communication: Evaluation of antibacterial activities and phytochemical composition of ethanolic extract of Diplazium esculentum. Biodiversitas, 25(3), 937-941.