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Sep 7, 2022
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Nov 9, 2022
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Dec 30, 2022
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Abstract

The Limau Sundai plant (Citrus Nobilis Lour) is widely known in West Sumatra. The fruit of this plant is commonly consumed as a cooking spice. This antioxidant activity test research aims to find out the potential of Lime Sundai peel as a source of antioxidant compounds. The peel of Limau Sundai fruit is ensnared with methanol solvent and concentrated using a rotary evaporator to obtain a coarse extract. The crude extract is then partitioned successively in n-hexane and ethyl acetate so that n-hexane, ethyl acetate, and methanol extracts are obtained. Sundai lime peel has good antioxidant activity against DPPH, which is shown in the ethyl fraction of acetate, methanol, and liquid methanol. The antioxidant activity of the three fractions is smaller than vitamin C and more significant than the hexane fraction. In the ethyl acetate, methanol and methanol/aqueous fractions with concentrations of 1076, 1822, and 1372 ppm in a row can reduce 50% of DPPH radical activity.

Keywords

Antioxidant, citrus nobilis lour, limau sundai, peels

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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1.
Mulia M, Efdi M, Afrizal A, Pernadi NL, Purnamasari D. Antioxidant Activities Extracts N-Hexane, Ethyl Acetate and Methanol of Limau Sundai (Citrus nobilis Lour) Peels. EKSAKTA [Internet]. 2022Dec.30 [cited 2024Apr.20];23(04):300-8. Available from: https://eksakta.ppj.unp.ac.id/index.php/eksakta/article/view/339

References

  1. D. R. Sultana, A. D. Shahin, and H. Md. Jawadul. (2022). Measurement of oxidative stress and total antioxidant capacity in hyperthyroid patients following treatment with carbimazole and antioxidant. Heliyon, vol. 8, no. 1, p. e08651.
  2. Mates, L., Popa, D. S., & Rusu, M. E. (2022). Walnut Intake Interventions Targeting Biomarkers of Metabolic Syndrome and Inflammation in Middle-Aged and Older Adults: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Antioxidants 2022, 11, 1412. Antioxidants in Age-Related Diseases and Anti-Aging Strategies, 239.
  3. Yang, Y., Liang, Y., Chen, J., Duan, X., & Guo, B. (2022). Mussel-inspired adhesive antioxidant antibacterial hemostatic composite hydrogel wound dressing via photo-polymerization for infected skin wound healing. Bioactive materials, 8, 341-354.
  4. Yang, J., Zhang, R., Zhao, H., Qi, H., Li, J., Li, J. F., ... & Zhang, T. (2022, August). Bioinspired copper single‐atom nanozyme as a superoxide dismutase‐like antioxidant for sepsis treatment. In Exploration (Vol. 2, No. 4, p. 20210267).
  5. V. Alagesan, S. Ramalingam, M. Kim, and S. Venugopal. (2019). Antioxidant activity guided isolation of a coumarin compound from Ipomoea pes-caprea (Convolvulaceae) leaves acetone extract and its biological and molecular docking studies. Eur. J. Integr. Med., vol. 32, p. 100984.
  6. Tolba, M., El-Dean, A., Geies, A., Radwan, S., Zaki, R., Sayed, M., ... & Abdel-Raheem, S. (2022). An overview on synthesis and reactions of coumarin based compounds. Current Chemistry Letters, 11(1), 29-42.
  7. K. Neha, M. R. Haider, A. Pathak, and M. S. Yar. (2019). Medicinal prospects of antioxidants: A review. Eur. J. Med. Chem., vol. 178, pp. 687–704.
  8. Sharaf, M. H., Abdelaziz, A. M., Kalaba, M. H., Radwan, A. A., & Hashem, A. H. (2022). Antimicrobial, antioxidant, cytotoxic activities and phytochemical analysis of fungal endophytes isolated from ocimum basilicum. Applied biochemistry and biotechnology, 194(3), 1271-1289.
  9. Ayoka, T. O., Ezema, B. O., Eze, C. N., & Nnadi, C. O. (2022). Antioxidants for the Prevention and Treatment of Non-communicable Diseases. Journal of Exploratory Research in Pharmacology, 7(3), 178-188.
  10. J. S. Agapin. (2020). Physico-Chemical Characterization of Essential Oil From Peel and Leaf of Dalanghita (Citrus Nobilis). SSRN Electron. J., vol. 4, no. 2, pp. 1–13.
  11. Huynh, P. X., Tran, N. T. H., Ly, D. T. T., Nguyen, T. N., & Le, T. D. (2022). Physiochemical properties, antibacterial, antifungal, and antioxidant activities of essential oils from orange (Citrus nobilis) peel. Emirates Journal of Food and Agriculture.
  12. Aini, N., Dwiyanti, H., Setyawati, R., Handayani, I., Septiana, A. T., Sustriawan, B., & Aena, D. A. Q. (2022). Siam orange (Citrus nobilis L.) nectar characteristics with variations in stabilizer and sucrose level. Food Res, 6(3), 315-323.
  13. S. Syafri, E. Husni, N. Wafiqah, F. Ramadhan, S. Ramadani, and D. Hamidi. (2020). Evaluation of Antimicrobial and Proliferation of Fibroblast Cells Activities of Citrus Essential Oils. Open Access Maced. J. Med. Sci., vol. 10, no. A, pp. 1051–1057.
  14. Sreepian, A., Popruk, S., Nutalai, D., Phutthanu, C., & Sreepian, P. M. (2022). Antibacterial activities and synergistic interaction of citrus essential oils and limonene with gentamicin against clinically isolated Methicillin-Resistant Staphylococcus aureus. The Scientific World Journal, 2022.
  15. Badalamenti, N., Bruno, M., Schicchi, R., Geraci, A., Leporini, M., Gervasi, L., ... & Loizzo, M. R. (2022). Chemical Compositions and Antioxidant Activities of Essential Oils, and Their Combinations, Obtained from Flavedo By-Product of Seven Cultivars of Sicilian Citrus aurantium L. Molecules, 27(5), 1580.
  16. W. Zhang, D. Liu, X. Fu, C. Xiong, and Q. Nie.(2022). Peel Essential Oil Composition and Antibacterial Activities of Citrus x sinensis L. Osbeck ‘Tarocco’ and Citrus reticulata Blanco. Horticulturae, vol. 8, no. 9.
  17. Qiu, L., Zhang, M., Adhikari, B., & Chang, L. (2022). Microencapsulation of rose essential oil in mung bean protein isolate-apricot peel pectin complex coacervates and characterization of microcapsules. Food Hydrocolloids, 124, 107366.
  18. H. C. Ko et al.. (2020). Changes in chemical composition and antioxidant activity of dried Citrus unshiu peel after roasting. Lwt, vol. 131, no. June, p. 109612.
  19. Dao, P. T., Tran, N. Y., Tran, Q. N., Bach, G. L., & Lam, T. V. (2022). Kinetics of pilot-scale essential oil extraction from pomelo (Citrus maxima) peels: Comparison between linear and nonlinear models. Alexandria Engineering Journal, 61(3), 2564-2572.
  20. R. Nawaz, N. A. Abbasi, I. A. Hafiz, Z. Khan, M. R. Khan, and A. Khalid. (2019). Impact Of Peel Coloration on Internal Quality f Kinnow Mandarin (Citrus nobilis Lour x Citrus deliciosa Tenora) at Early Ripening Stage. World J. Biol. Biotechnol., vol. 4, no. 1.
  21. Dao, T. P., Tran, N. Q., & Tran, T. T. (2022). Assessing the kinetic model on extraction of essential oil and chemical composition from lemon peels (Citrus aurantifolia) by hydro-distillation process. Materials Today: Proceedings, 51, 172-177.
  22. M. Mulia. (2017). Isolasi Kumarin Dari Kulit Buah Limau Sundai (Citrus nobilis Lour). EKSAKTA Berk. Ilm. Bid. MIPA, vol. 18, no. 02, pp. 137–145.
  23. Oulebsir, C., Mefti-Korteby, H., Djazouli, Z. E., Zebib, B., & Merah, O. (2022). Essential Oil of Citrus aurantium L. Leaves: Composition, antioxidant activity, elastase and collagenase inhibition. Agronomy, 12(6), 1466.
  24. Wei, L., Pu, D., Mi, S., Yang, H., Wei, L., Lu, Q., ... & Zu, Y. (2022). Essential oil extraction and evaluation from the fresh Platycladus orientalis (L.) Franco seed peel waste by an environment-friendly method. Sustainable Chemistry and Pharmacy, 29, 100771.
  25. Xin, K. Q., Ji, X. Y., Guo, Z., Han, L., Yu, Q. L., & Hu, B. (2022). Pitaya peel extract and lemon seed essential oil as effective sodium nitrite replacement in cured mutton. LWT, 160, 113283.
  26. C. Di Vaio, G. Graziani, A. Gaspari, G. Scaglione, S. Nocerino, and A. Ritieni. (2010). Essential oils content and antioxidant properties of peel ethanol extract in 18 lemon cultivars. Sci. Hortic. (Amsterdam)., vol. 126, no. 1, pp. 50–55.
  27. Khamsaw, P., Lumsangkul, C., Karunarathna, A., Onsa, N. E., Kawichai, S., Chuttong, B., & Sommano, S. R. (2022). Recovery of Orange Peel Essential Oil from ‘Sai-Namphaung’ Tangerine Fruit Drop Biomass and Its Potential Use as Citrus Fruit Postharvest Diseases Control. Agriculture, 12(5), 701.
  28. A. E. Hegazy and M. I. Ibrahium. (2012). Antioxidant activities of orange peel extracts. World Appl. Sci. J., vol. 18, no. 5, pp. 684–688.
  29. Badalamenti, N., Bruno, M., Schicchi, R., Geraci, A., Leporini, M., Gervasi, L., ... & Loizzo, M. R. (2022). Chemical Compositions and Antioxidant Activities of Essential Oils, and Their Combinations, Obtained from Flavedo By-Product of Seven Cultivars of Sicilian Citrus aurantium L. Molecules, 27(5), 1580.
  30. J. H. Park, M. Lee, and E. Park. (2014). Antioxidant activity of orange flesh and peel extracted with various solvents. Prev. Nutr. Food Sci., vol. 19, no. 4, pp. 291–298.
  31. Tong, X., Cao, J., Tian, T., Lyu, B., Miao, L., Lian, Z., ... & Jiang, L. (2022). Changes in structure, rheological property and antioxidant activity of soy protein isolate fibrils by ultrasound pretreatment and EGCG. Food Hydrocolloids, 122, 107084.
  32. N. Aini et al. (2022). Siam orange (Citrus nobilis L.) nectar characteristics with variations in stabilizer and sucrose level. Food Res., vol. 6, no. 3, pp. 315–323.
  33. G. S. Pereira, A. R. Honorio, B. R. Gasparetto, C. M. A. Lopes, D. C. N. d. Lima, and A. A. L. Tribst. (2019). Influence of information received by the consumer on the sensory perception of processed orange juice. J. Sens. Stud., vol. 34, no. 3, pp. 1–12.
  34. Souri, E., Amin, G., Farsam, H., Jalalizadeh, H., & Barezi, S. (2022). Screening of thirteen medicinal plant extracts for antioxidant activity. Iranian Journal of Pharmaceutical Research, 7(2), 149-154.
  35. Roy, S., Ezati, P., Rhim, J. W., & Molaei, R. (2022). Preparation of turmeric-derived sulfur-functionalized carbon dots: antibacterial and antioxidant activity. Journal of Materials Science, 57(4), 2941-2952.
  36. M. M. Hasan, P. Roy, M. Alam, M. M. Hoque, and W. Zzaman. (2022). Antimicrobial activity of peels and physicochemical properties of juice prepared from indigenous citrus fruits of Sylhet region, Bangladesh, Heliyon, vol. 8, no. 7, p. e09948.
  37. Hossain, M. N., Sarker, U., Raihan, M. S., Al-Huqail, A. A., Siddiqui, M. H., & Oba, S. (2022). Influence of salinity stress on color parameters, leaf pigmentation, polyphenol and flavonoid contents, and antioxidant activity of Amaranthus lividus leafy vegetables. Molecules, 27(6), 1821.
  38. A. Malik, A. Najda, A. Bains, R. Nurzyńska-Wierdak, and P. Chawla. (2021). Characterization of citrus nobilis peel methanolic extract for antioxidant, antimicrobial, and anti-inflammatory activity, Molecules, vol. 26, no. 14.
  39. Ezati, P., Rhim, J. W., Molaei, R., Priyadarshi, R., Roy, S., Min, S., ... & Han, S. (2022). Preparation and characterization of B, S, and N-doped glucose carbon dots: Antibacterial, antifungal, and antioxidant activity. Sustainable Materials and Technologies, 32, e00397.
  40. Sheng, Y., Ma, Z., Wang, X., & Han, Y. (2022). Ethanol organosolv lignin from different agricultural residues: Toward basic structural units and antioxidant activity. Food Chemistry, 376, 131895.
  41. H. C. Himawan, A. F. Isa, and D. S. Wiharja. (2021). Antioxidant Activity of 70% Ethanol Extract Combination of Kemangi Leaf (Ocimum Americanum Linn) and Binahong Leaf (Anredera cordifolia (Ten.) Steenis) Using DPPH, J. Phys. Conf. Ser., vol. 1764, no. 1.
  42. Andriopoulos, V., Gkioni, M. D., Koutra, E., Mastropetros, S. G., Lamari, F. N., Hatziantoniou, S., & Kornaros, M. (2022). Total phenolic content, biomass composition, and antioxidant activity of selected marine microalgal species with potential as aquaculture feed. Antioxidants, 11(7), 1320.
  43. S. Baliyan et al. (2022). Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religios. Molecules, vol. 27, no. 4, p. 1326.
  44. Marchi, R. C., Campos, I. A., Santana, V. T., & Carlos, R. M. (2022). Chemical implications and considerations on techniques used to assess the in vitro antioxidant activity of coordination compounds. Coordination Chemistry Reviews, 451, 214275.
  45. A. S. Daulay, Ridwanto, R. A. Syahputra, and A. Nafitri. (2021). Antioxidant Activity Test of Chayote (Sechium edule (Jacq.) Swartz) Ethanol Extract using DPPH Method. J. Phys. Conf. Ser., vol. 1819, no. 1.
  46. Huang, D., Li, C., Chen, Q., Xie, X., Fu, X., Chen, C., ... & Dong, H. (2022). Identification of polyphenols from Rosa roxburghii Tratt pomace and evaluation of in vitro and in vivo antioxidant activity. Food Chemistry, 377, 131922.
  47. N. Rai et al. (2022). Bioprospecting of fungal endophytes from Oroxylum indicum (L.) Kurz with antioxidant and cytotoxic activity. PLoS One, vol. 17, no. 3, p. e0264673.
  48. Tamfu, A. N., Ceylan, O., Cârâc, G., Talla, E., & Dinica, R. M. (2022). Antibiofilm and anti-quorum sensing potential of cycloartane-type triterpene acids from cameroonian grassland propolis: phenolic profile and antioxidant activity of crude extract. Molecules, 27(15), 4872.
  49. M. D. Hidayati, T. Ersam, K. Shimizu, and S. Fatmawati. (2017). Antioxidant activity of Syzygium polynthum extracts. Indones. J. Chem., vol. 17, no. 1, pp. 49–53.
  50. Shen, N., Wang, T., Gan, Q., Liu, S., Wang, L., & Jin, B. (2022). Plant flavonoids: Classification, distribution, biosynthesis, and antioxidant activity. Food Chemistry, 132531.
  51. E. Husni, F. Ismed, and Awaliana. (2021). Standardization extracts and simplicia of limau sundai peel (Citrus x aurantiifolia ’sundai’), determine content of nobiletin and antibacterial activity test. Pharmacogn. J., vol. 13, no. 6, pp. 1323–1331.
  52. X. Kui et al. (2020). Comparison of physico-chemical characteristics of myrtle at different ripening stages. Folia Hortic., vol. 32, no. 2, pp. 125–133.
  53. I. B. Tanjung, N. N. Azizah, A. Arsianti, A. S. Anisa, and K. A. Audah. (2022). Evaluation of the Ethyl Acetate Extract of the Roots of Avicennia marina as Potential Anticancer Drug. Proc. 6th Int. Conf. Food, Agric. Nat. Resour. (IC-FANRES 2021), vol. 16, no. Figure 1, pp. 75–81.
  54. G. K. Jayaprakasha, B. Girennavar, and B. S. Patil. (2008). Antioxidant capacity of pummelo and navel oranges: Extraction efficiency of solvents in sequence. LWT - Food Sci. Technol., vol. 41, no. 3, pp. 376–384.
  55. R. Mustarichie, D. Runadi, and D. Ramdhani. (2017). The antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea BL. dans). Asian J. Pharm. Clin. Res., vol. 10, no. 2, pp. 343–347.
  56. Hu, X., Wu, D., Tang, L., Zhang, J., Zeng, Z., Geng, F., & Li, H. (2022). Binding mechanism and antioxidant activity of piperine to hemoglobin. Food Chemistry, 394, 133558.

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