Roselle (Hibiscus sabdariffa L.) Tea Protects Against Alcohol-Induced Renal Toxicity by Enhancing Endogenous Antioxidant Defence in Wistar Rats

Putri Dafriani; Eliza Arman; Annita Annita

doi:10.24036/eksakta/vol27-iss03/688

EKSAKTA: Berkala Ilmiah Bidang MIPA

Open Access

Roselle (Hibiscus sabdariffa L.) Tea Protects Against Alcohol-Induced Renal Toxicity by Enhancing Endogenous Antioxidant Defence in Wistar Rats

Putri Dafriani ¹

, Eliza Arman ²

, Annita Annita ²

¹ Department of Nursing, Syedza Saintika University, Padang, Indonesia

² Department of Medical Laboratory Technology, Syedza Saintika University, Padang, Indonesia

Published 2026-06-30

Vol. 27 No. 03 (2026) PAGES 257-264

DOI 10.24036/eksakta/vol27-iss03/688

Views / Downloads 19 24

Download PDF

Abstract

In Indonesia, the choice between area source and smoothed seismicity approaches has been driven by data availability rather than scientific evaluation, and no systematic comparison of both methods at the national scale has been conducted. This study compared both approaches across Indonesia to evaluate their differences in PGA estimation and assess the forecasting skill of the smoothed seismicity model. Both models were applied to the same declustered earthquake catalog with a Magnitude of Completeness of 4.7, b-value of 0.89, and a-value of 8.753, using identical Ground Motion Prediction Equations within a probabilistic seismic hazard analysis framework. Both models consistently identified North Sulawesi as the highest hazard region and Kalimantan as the lowest. The smoothed seismicity model produced higher maximum PGA values of 2.13 g compared to 1.1597 g and 1.5901 g from the area source model at 10% and 2% probability of exceedance in 50 years, respectively. Molchan Diagram validation yielded an Area Skill Score of 0.88 and R-score of 0.656, confirming strong forecasting skill. The two methods are complementary, and their integration within a logic tree framework is recommended for future national-scale PSHA in Indonesia.

References

1

[1] Bryazka, D., Reitsma, M. B., Griswold, M. G., Abate, K. H., Abbafati, C., Abbasi-Kangevari, M., ... & Diress, M. (2022). Population-level risks of alcohol consumption by amount, geography, age, sex, and year: a systematic analysis for the Global Burden of Disease Study 2020. The Lancet, 400(10347), 185-235.

2

[2] Bai, H. J., Wang, Y. W., Li, Z. Q., Lu, D. X., Nan, X. M., & Yang, Z. T. (2026). Perillyl alcohol attenuates hypoxia induced right ventricular dysfunction and remodeling by balancing the renin angiotensin aldosterone system in rats. Scientific Reports.

3

[3] Contreras-Zentella, M. L., Villalobos-García, D., & Hernández-Muñoz, R. (2022). Ethanol metabolism in the liver, the induction of oxidant stress, and the antioxidant defense system. Antioxidants, 11(7), 1258.

4

[4] Hosseini, N. S., Shirazpour, S., Sepehri, G., Dabiri, S., & Meymandi, M. S. (2025). High-intensity interval training alleviates ethanol-induced renal damage: A study on inflammation, oxidative stress, and histopathological changes in rats. Drug and Alcohol Dependence Reports, 14, 100320.

5

[5] Efosa, J. O., Omage, K., & Azeke, M. A. (2023). Hibiscus sabdariffa calyx protect against oxidative stress and aluminium chloride-induced neurotoxicity in the brain of experimental rats. Toxicology reports, 10, 469-480.

6

[6] Tsermpini, E. E., Plemenitaš Ilješ, A., & Dolžan, V. (2022). Alcohol-induced oxidative stress and the role of antioxidants in alcohol use disorder: a systematic review. Antioxidants, 11(7), 1374.

7

[7] Mirghaed, A. T., Khoshkharam, N., Karimi, F., Abdzadeh, S., Razazan, M., Rahami, Z., & Doustimotlagh, A. H. (2025). Nephroprotective effects of ethanol leaf extract of Stachys pilifera Benth in alcohol-induced nephrotoxicity in male rats. Research in Pharmaceutical Sciences, 20(5), 723-733.

8

[8] Kiani, M., Mirzaei, H., Enayati, A., Ghorbani, S., Zengin, G., & Amirkhanlou, S. (2026). Comparison of Hibiscus sabdariffa L. extract and hydrochlorothiazide as adjuncts to Valsartan in managing hypertension in type 2 diabetic nephropathy: A randomized clinical trial. Avicenna Journal of Phytomedicine, 16(2), 136.

9

[9] Szada-Borzyszkowski, K., Owczarenko, K., Wróbel, B., Wójcik, L., Filipski, M., & Gadzalski, K. (2026). Impact of Hibiscus sabdariffa supplementation on human health: a narrative review. Journal of Education, Health and Sport, 89, 70362-70362.

10

[10] Ajiboye, B. O., Famusiwa, C. D., Nifemi, D. M., Ayodele, B. M., Akinlolu, O. S., Fatoki, T. H., ... & Oyinloye, B. E. (2024). Nephroprotective effect of Hibiscus Sabdariffa leaf flavonoid extracts via KIM-1 and TGF-1β signaling pathways in streptozotocin-induced rats. ACS omega, 9(17), 19334-19344.

11

[11] Adusei, S. (2020). Bioactive compounds and antioxidant evaluation of methanolic extract of Hibiscus sabdariffa. IPTEK the Journal for Technology and Science, 31(2), 139-147.

12

[12] Mhya, D. H., Nwabugo, S., Sambo, M., & Mankilik, M. M. (2026). In silico, in vitro and in vivo evaluation of anti-inflammatory chemicals from Hibiscus sabdariffa and Citrus paradisi alone and in combination. Biokemistri, 38(1), 53-73.

13

[13] Alyani, F. S., Yulianti, R., & Thadeus, M. S. (2021). The effect of roselle (Hibiscus sabdariffa) extract on malondialdehyde level in rat liver. Jurnal Gizi Dan Pangan, 16(1), 57-62.

14

[14] Ajiboye, B. O., Famusiwa, C. D., Falode, J. A., Akojuru, D. O., Owolabi, B. T., Adejumo, A. A., ... & Ojo, O. A. (2025). Protective effects of flavonoid-rich extracts from Hibiscus sabdariffa leaves on streptozotocin-induced testicular damage in rats. Phytomedicine Plus, 5(2), 100808.

15

[15] Ujianti, I., Sianipar, I. R., Prijanti, A. R., & Santoso, D. I. S. (2022). Consumption of hibiscus sabdariffa dried calyx ethanol extract improved redox imbalance and glucose plasma in vitamin b12 restriction diet in rats. Malaysian Applied Biology, 51(2), 33-40.

16

[16] Muhamad Rosli, S. H., Lim, X. Y., Krishnan, P., Ahmad, I. F., Voon, Y. L., Siau, T. C., ... & Tan, T. Y. C. (2026). Unveiling the safety, tolerability, and herb-drug interaction concerns of Hibiscus Sabdariffa L.(Roselle): A systematic scoping review of current evidence. Heliyon, 12(3), e44583.

17

[17] Guerassimoff, L., & Nicolas, J. (2026). Towards safer medicines: Management of residual solvents and green alternatives. Journal of Controlled Release, 114643.

18

[18] Mostafa, A. E. (2025). Pharmacological Safety Profiling of Nigella sativa (NS) and Hibiscus sabdariffa (HS) Extracts in Zebrafish (Danio rerio) Embryos: An Integrative Vet-Pharmacotoxicological Evaluation of LC₅₀, Teratogenicity, and Developmental Outcomes. Mansoura Veterinary Medical Journal, 26(2), 3.

19

[19] Sireeratawong, S., Itharat, A., Khonsung, P., Lertprasertsuke, N., & Jaijoy, K. (2013). Toxicity studies of the water extract from the calyces of Hibiscus sabdariffa L. in rats. African Journal of Traditional, Complementary, and Alternative Medicines, 10(4), 122.

20

[20] Koyama, Y., Kobayashi, Y., Hirota, I., Kobayashi, H., & Shimada, S. (2026). Silicon-based agent mitigates fatty liver formation in a CDAHFD60-induced MASH mouse model by enhancing hepatic function. Biochemistry and Biophysics Reports, 46, 102552.

21

[21] Tjitraresmi, A., Febriyanti, R. M., Anjabtsawa, D., Susilawati, Y., & Muhaimin, M. (2025). Antidiabetic activity of combined extracts of Hibiscus sabdariffa Linn. and Stevia rebaudiana Bert. on streptozotocin-induced diabetes Wistar rats. Chempublish Journal, 9(2), 167-182.

22

[22] de Lira Alencar, N. R. C., Figueiredo Neto, A., Queiroz, M. A. Á., de Carvalho, F. A. L., Viana, A. C., da Silva Júnior, E. B., ... & Rodrigues, R. T. D. S. (2026). Effects of Hibiscus rosa-sinensis flower powder as a natural colorant and antioxidant on the physicochemical and sensory properties of frozen lamb burgers. Journal of Food Science and Technology, 1-12.

23

[23] Niu, L., Luo, Y., Xie, W., Wang, C., & Liu, Z. (2026). Dietary (−)-Epigallocatechin Gallate (EGCG): State-of-the-Art Advances in Bioactivities, Bioavailability Enhancement Strategies, and Applications in Nutrition and Health. Nutrients, 18(2), 317.

24

[24] Vydani, K., Sadanala, S. V. D., Ganta, B., Nemala, N. S., Dandingi, T. S., & Karri, S. (2026). A Review on Extraction, Pharmacological Potential and Therapeutic Applications of Hibiscus rosa-sinensis. Journal of Pharma Insights and Research, 4(1), 169-177.

25

[25] Pacifico, S., Caputo, E., Piccolella, S., Diglio, C., Zenone, T., Bertolini, T., & Mandrich, L. (2026). Exploiting Vegetarian and Vegan Cheeses with Hibiscus Extract: Functional and Nutritional Perspectives. Future Foods, 100981.

26

[26] Djabir, Y. Y., Adnan, J., Amalia, N., Ramli, N., Sartini, S., Mamada, S. S., & Usmar, U. (2021). Roselle (Hibiscus sabdariffa L.) calyx water extract ameliorates isoniazid and rifampicin induced liver and renal injuries in rats. Journal of Herbmed Pharmacology, 10(3), 296-303.

27

[27] Chukwu, C. N., Chukwu, C., Ogunka-Nnoka, C. U., & Ajah, O. (2026). Comparative Evaluation of Nutritional, Phytochemical, and Techno-Functional Properties of Raw, Boiled, and Fermented Hibiscus sabdariffa Seeds. Food and Humanity, 101034.

28

[28] Prihastuti, A. E., Nurudin, E., Safitri, A., Noviatri, A., Widyaputri, T., & Sari, C. (2025). Kadar Blood Urea Nitrogen dan Kreatinin Tikus Model Toksisitas Rhodamin B dan Sakarin yang Disuplementasi Yogurt Rosela Ungu. Indonesia Medicus Veterinus, 14(2), 90-100.

29

[29] Hosseini, N. S., Shirazpour, S., Sepehri, G., Dabiri, S., & Meymandi, M. S. (2025). High-intensity interval training alleviates ethanol-induced renal damage: A study on inflammation, oxidative stress, and histopathological changes in rats. Drug and Alcohol Dependence Reports, 14, 100320.

30

[30] Regalado-Rentería, E., Serna-Tenorio, J. E., García-Gutiérrez, D. G., Reynoso-Camacho, R., Anaya-Loyola, M. A., & Pérez-Ramírez, I. F. (2026). Dietary Intervention with Hibiscus sabdariffa L. Beverage Residue Attenuates Dyslipidemia and Hepatic Steatosis in Late-Stage Type 2 Diabetic Rats. Nutraceuticals, 6(2), 23.

31

[31] Dehkhoda, B., Enayati, A., Mirzaei, H., Ghorbani, S., Soleimani, M. H., Amirkhanlou, S., & Sahebkar, A. (2024). Roselle (Hibiscus sabdariffa L.) extract as an adjunct to valsartan in patients with mild chronic kidney disease: a double-blind randomized controlled clinical trial. Avicenna journal of phytomedicine, 14(4), 505.

32

[32] Kouémou, N. E., Sepi, L. A., Dongmo, M. S. N., Tamanji, N. L., Mbeboh, F. S., Ndjapdounke, S. J. K., ... & Bum, E. N. (2026). Hibiscus sabdariffa calyx aqueous extract mitigates alcohol withdrawal-induced anxiety and oxidative stress in mice. Biochemistry and Biophysics Reports, 45, 102423.

33

[33] Okafor, I. A., Mbagwu, S. I., Okafor, U. S., Nweke, J. O., Ibeabuchi, K. C., & Ogbonna, S. N. (2023). The methanolic extract of Hibiscus sabdariffa downregulates the relative expression of Kiss1 gene in the hypothalamus of Wistar rats: A preliminary report. Avicenna Journal of Phytomedicine, 13(6), 575.

Roselle (Hibiscus sabdariffa L.) Tea Protects Against Alcohol-Induced Renal Toxicity by Enhancing Endogenous Antioxidant Defence in Wistar Rats

Abstract

References

How to Cite

Cite Article

Article Update