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Abstract

Cassava (Manihot esculenta) is one of the food crops that contain high carbohydrate and is used as a raw material for various industries. Cassava also contains several types of proteins, including beta-carotene. However, some plants also contain various levels of beta-carotene. Beta-carotene is one of the groups of carotenoids that serve as precursors of vitamin A. This bioinformatics study aims to determine the similarities and differences in the enzyme encoding genes involved in carotenoid biosynthesis pathways in cassava with several plants containing beta-carotene from nucleotide sequences and encoding gene proteins carotenoids (Lcy α and Lcy β). Data on nucleotide and protein sequences were obtained through the NCBI database. Similarities and differences in nucleotide and protein sequence data are seen from the phylogenetic tree using the MEGA 7.0 program. Based on the nucleotide gene Lcy α Manihot esculenta with several plants containing beta-carotene is not related. Based on the phylogenetic tree nucleotide sequence protein Lcy α and Lcy β Manihot esculenta with several plants containing beta-carotene there were differences in similarity

Keywords

Beta-carotene Lcy α and Lcy β bioinformatics Manihot esculenta Ub Manihot esculenta Beta-caroten Lcy α dan Lcy β bioinformatik

Article Details

How to Cite
1.
Putri DH, Anika M, Wahyuni W. Bioinformatics Study Genes Encoding Enzymes Involved in the Biosynthesis of Carotenoids Line Cassava (Manihot esculenta). Eksakta [Internet]. 2019Apr.30 [cited 2021Jul.25];20(1):10-6. Available from: https://eksakta.ppj.unp.ac.id/index.php/eksakta/article/view/161

References

  1. Bendich, A. . 1990. Carotenoids y el sistema inmune, Química y biología: 323-35.
  2. Britton, G. . 1995. Structure and properties of carotenoids in relation to function, FASEB J, 9: 1551– 58.
  3. Cameron, K. M. . 2005. Leave it to the Leaves: a Molecular Phylogenetic Study of Malaxideae (Epidendroideae, Orchidaceae), Am. J. Bot., 92: 1025-32.
  4. Carvalho, L. J., Marco AV Agustini, James V Anderson, Eduardo A Vieira, Claudia RB de Souza, Songbi Chen, Barbara A Schaal and Joseane P Silva. 2016. Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root BMC Plant Biology, 16: 133.
  5. Eddy, N. Q., Essien, E., Ebenso, E. E., dan Ukpe, R. A. 2012. Industrial Potential of Two Varieties of Cocoyam in Bread Making, J. Chem., 9: 451-64.
  6. Hartati, N. Sri, Hani Fitriani, Supatmi, Enny Sudarmonowati. 2012. Karakter Umbi Dan Nutrisi Tujuh Genotip Ubi Kayu (Manihot esculenta), Jurnal Agricola, 2.
  7. Hasyim, Ashol dan M. Yusuf. 2007. Ubi Jalar Kaya Antosianin Pilihan Pangan Sehat. In www.puslittan.bogor.net. Bogor: Puslitbangtan.
  8. Karnjanawipagul, P. W. Nittayanuntawech, P. Rojsanga and L. Suntornsuk. 2010. Analysis of β-Carotene in Carrot by Spectrophotometry, Mahidol University Journal of Pharmaceutical Science, 37: 8-16.
  9. Prabhala, R. H., H.S. Garewal, F.L. Meyskens, and R.R. Watson. 1990. Modulación de Immuno en humanos causado por caroteno y vitamina A, Nutr. Resolución, 10: 1473-86.
  10. Purwono. 2009. Klasifikiasi Pengelompokan Ubi kayu (Balai Besar Penelitian Pengembangan Pasaca Panen: Bogor).
  11. Stansfield, William, Raul Cano, and Jaime Colome. 2006. Schaum's Easy Outlines: Biologi Molekuler dan Sel (Erlangga: Jakarta).
  12. Suyamto, S. d. M. . 2009. Kesiapan Teknologi Mendukung Peningkatan Produksi Kedelai dan Ubi Kayu, Prosiding Inovasi Teknologi kacang-kacangan dan umbi-umbian mendukung kemandirian pangan dan kecukupan energi, 16: 50.
  13. Vinolina, N. S. . 2009. Biosintesis Senyawa Karotenoid, Jurnal Penelitian Bidang Ilmu Pertanian, 7: 148 – 54.
  14. Yuwono. 2005. Biologi Molekular (Erlangga: Jakarta).