Article Sidebar

Submitted
Feb 24, 2026
Accepted
Apr 1, 2026
Published
Apr 8, 2026
Download
pdf
Statistic
Read Counter : 16 Download : 3

Downloads

Download data is not yet available.

Main Article Content

Abstract

Thrombapheresis is commonly used to obtain platelet concentrates; however, different collection doses may affect donor hematological responses. This study aimed to compare the effects of single-dose and double-dose thrombapheresis on platelet count, thrombopoietin (TPO) levels, and immature platelet fraction (IPF). A prospective comparative study with repeated measures was conducted in eight healthy donors (n = 8; 4 per group) at the Indonesian Red Cross Blood Donation Unit, Tangerang. Blood samples were collected before, immediately after, and on day 2 and day 4 post-donation. Platelet count and IPF were measured using an automated hematology analyzer, while TPO levels were assessed by ELISA. Data were analyzed using two-way ANOVA and Pearson correlation (p < 0.05). Platelet counts decreased immediately after thrombapheresis (23% in single-dose; 24% in double-dose) and gradually recovered. Significant effects of time and dose were observed (p < 0.05), but no difference between groups at day 4 (p > 0.05). TPO increased in both groups (50% vs 27%), and IPF changes were not significant (p > 0.05). A positive but non-significant correlation between TPO and IPF was found. Both procedures cause transient platelet reduction with gradual recovery. Findings should be interpreted cautiously due to the small sample size.

Keywords

Platelet Thrombopoietin Platelet apheresis Platelet count

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite
1.
Aulia Ramadhan Supit, Anzhari DH, Saptuti Chunaeni, Rahajuningsih Dharma Setiabudy. The Effect of Single and Double Dose Platelet Apheresis on Platelet Count A Thrombopoietin Levels and Percentage of Immature Platelet Fraction of Donor Post Donation. EKSAKTA [Internet]. 2026 Apr. 8 [cited 2026 Apr. 11];27(02):132-43. Available from: https://eksakta.ppj.unp.ac.id/index.php/eksakta/article/view/663

References

  1. [1] Agarwal, A., Khan, A. I., & Anwer, F. (2024). Platelet transfusion. In StatPearls [Internet]. StatPearls Publishing.
  2. [2] Allard, S. (2009). Blood transfusion. Medicine, 37(3), 172-176.
  3. [3] Carson, J. L., Stanworth, S. J., Guyatt, G., Valentine, S., Dennis, J., Bakhtary, S., ... & Pagano, M. B. (2023). Red blood cell transfusion: 2023 AABB international guidelines. Jama, 330(19), 1892-1902.
  4. [4] Statistik, B. P. (2025). Statistik Indonesia 2025: Statistical yearbook of Indonesia 2025 (Vol. 53). Badan Pusat Statistik.
  5. [5] Aji Muhawarman. (2025). Kasus Kanker Diprediksi Meningkat 70 Persen pada 2050, Kemenkes Perkuat Deteksi Dini
  6. [6] Aji Muhawarman. (2025). Nyamuk lebih mematikan daripada hewan buas. Kementerian Kesehatan Republik Indonesia
  7. [7] Lee, E. J., & Lee, A. I. (2016). Thrombocytopenia. Primary Care: Clinics in Office Practice, 43(4), 543-557.
  8. [8] Ramaiah, L., Erkens, T., Sirivelu, M., & Vitsky, A. (2025). Hematopoietic system. In Haschek and Rousseaux's handbook of Toxicologic pathology (pp. 337-436). Academic Press.
  9. [9] Kaushansky, K. (2024). Thrombopoietin, the primary regulator of platelet production: from mythos to logos, a thirty-year journey. Biomolecules, 14(4), 489.
  10. [10] Gauer, R. L., & Braun, M. M. (2012). Thrombocytopenia. American family physician, 85(6), 612-622.
  11. [11] Jeon, K., Kim, M., Lee, J., Lee, J. S., Kim, H. S., Kang, H. J., & Lee, Y. K. (2020). Immature platelet fraction: a useful marker for identifying the cause of thrombocytopenia and predicting platelet recovery. Medicine, 99(7), e19096.
  12. [12] Asghar, M. B., Akhtar, F., Mahmood, A., Rafique, N., Rana, N. A., & Khalid, U. B. (2023). Diagnostic accuracy of immature platelet fraction (IPF) to differentiate between thrombocytopenia due to peripheral destruction versus bone marrow failure. Age (years), 27(19.1), 30-24.
  13. [13] Ali, I., Graham, C., & Dempsey-Hibbert, N. C. (2019). Immature platelet fraction as a useful marker in the etiological determination of thrombocytopenia. Experimental Hematology, 78, 56-61.
  14. [14] Kaufman, R. M., Djulbegovic, B., Gernsheimer, T., Kleinman, S., Tinmouth, A. T., Capocelli, K. E., ... & Tobian, A. A. (2015). Platelet transfusion: a clinical practice guideline from the AABB. Annals of internal medicine, 162(3), 205-213.
  15. [15] Metcalf, R. A., Nahirniak, S., Guyatt, G., Bathla, A., White, S. K., Al-Riyami, A. Z., ... & Stanworth, S. J. (2025). Platelet transfusion: 2025 AABB and ICTMG international clinical practice guidelines. JAMA, 334(7).
  16. [16] Das SS, Sen S, Zaman RU, Biswas RN. (2021). Plateletpheresis in the Era of Automation: Optimizing Donor Safety and Product Quality Using Modern Apheresis Instruments. Indian Journal of Hematology and Blood Transfusion ;37:134–9. https://doi.org/10.1007/s12288-020-01337-1.
  17. [17] Hans, R., Sharma, R. R., & Marwaha, N. (2019). Effect of plateletpheresis on postdonation serum thrombopoietin levels and its correlation with platelet counts in healthy voluntary donors. Asian Journal of Transfusion Science, 13(1), 10-16.
  18. [18] Indonesia, M. K. R. (2015). Standar Pelayanan Transfusi Darah. Pelayanan Transfusi Darah.
  19. [19] Chopra, S., Kaur, P., Bedi, R. K., & Kaur, G. (2023). Effect of double dose plateletpheresis on target yield and donor platelet recovery. Hematology, Transfusion and Cell Therapy, 45(1), 16-24.
  20. [20] Makroo, R. N., Fadadu, D., Agrawal, S., & Chowdhry, M. (2018). Double dose plateletpheresis: a savior to shrinking donor pool and platelet inventory management. Indian Journal of Hematology and Blood Transfusion, 34(4), 691-696.
  21. [21] Thokala, R. P., Radhakrishnan, K., Anandan, A., & Panicker, V. K. (2016). Recovery of platelet count among apheresis platelet donors. Journal of clinical and diagnostic research: JCDR, 10(12), EC01.
  22. [22] Rajput, S., Makroo, R. N., Chowdhry, M., & Thakur, U. K. (2021). Changes in hematological parameters post plateletpheresis: Single center study from North India. Transfusion and Apheresis Science, 60(6), 103283.
  23. [23] Patel, K. B., Inaniya, K., & Padharia, D. P. (2023). Recovery period for attaining baseline haematological parameters after plateletpheresis donation-a cohort study. J Clin Diagn Res, 17(4).
  24. [24] Jiang, H., Jin, Y., Shang, Y., Yuan, G., Liu, D., Li, J., ... & Zhou, F. (2021). Therapeutic plateletpheresis in patients with thrombocytosis: gender, hemoglobin before apheresis significantly affect collection efficiency. Frontiers in Medicine, 8, 762419.
  25. [25] Elsayed, A., Elsayed, B., Elmarasi, M., Elsabagh, A. A., Elsayed, E., Elmakaty, I., & Yassin, M. (2024). Thrombopoietin receptor agonists in post-hematopoietic cell transplantation complicated by prolonged thrombocytopenia: a comprehensive review. ImmunoTargets and Therapy, 461-486.
  26. [26] Hans, R., Sharma, R. R., & Marwaha, N. (2019). Effect of plateletpheresis on postdonation serum thrombopoietin levels and its correlation with platelet counts in healthy voluntary donors. Asian Journal of Transfusion Science, 13(1), 10-16.
  27. [27] Rosyidah, R. A., Anjani, N., Hartini, W. M., & Mardiyaningsih, A. (2023). Perbedaan Jumlah Trombosit Pasca Transfusi Thrombocyte Concentrate Dan Thrombocyte Apheresis Pada Pasien Trombositopenia. Jurnal Kesehatan, 11(1), 169-182.
  28. [28] Kariyawasan, C. C., Botenne, C. S., Balasuriya, B. L. T., Ruhunuhewa, U. S., Dissanayake, D. M. C., & Ranatunga, S. A. C. D. (2024). Diagnostic Utility of Immature Platelet Fraction (IPF) in Differentiating Thrombocytopenia Due to Increased Thrombopoietic Activity. Achievements and Challenges of Medicine and Medical Science Vol. 9, 60-73.
  29. [29] Ding, S., Wang, M., Fang, S., Xu, H., Fan, H., Tian, Y., ... & Sun, X. (2025). Corrigendum: D-dencichine regulates thrombopoiesis by promoting megakaryocyte adhesion, migration and proplatelet formation. Frontiers in Pharmacology, 15, 1504076.
  30. [30] Bajaj, H., Rajpal, T., Sharma, M., Singh, P., Hemal, A., & Kumar, V. (2024). Role of immature platelet fraction in etiological diagnosis of thrombocytopenia. Journal of Laboratory Physicians, 16(4), 496-500.
  31. [31] Zhu, M., Sharma, P., Mete, M., Olcal, A., Ibrahim, I., Chen, W., & Bat, T. (2025). Absolute Immature Platelet Count: An Accessible Biomarker to Distinguish Aplastic Anemia and Immune Thrombocytopenia. International Journal of Laboratory Hematology, 47(2), 357-361.