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Dec 1, 2024
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Feb 1, 2025
Published
Feb 11, 2025
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Abstract

Central Kalimantan is rich in natural resources of potential medicinal plants, such as bay leaf and noni. These medicinal plants have been used traditionally. However, there is minimal scientific evidence about their use as a medicine due to limited research. The current research aims to identify the influence of applying a combination of bay leaf and noni fruit extract in lowering cholesterol levels in Balb/c hypercholesterolemic mice. The research employs a mixed method, namely explorative and experimental research. The research subjects are 28 female Balb/c mice in a hypercholesterolemic condition. The research consists of 7 treatments, namely positive control, negative control, P1 (20%), P2 (30%), P3 (40%), P4 (50%), and P5 (60%) with 4x repetitions. It combines the extract of bay leaf and noni fruit to lower cholesterol levels in the research subjects. Data analysis uses one-way ANOVA. The research results indicate that the application of a combination of bay leaf and noni fruit extract has a significant influence on the reduction of cholesterol levels in hypercholesterolemic mice with a p-value α (α 0.05) of 0.000. The concentration effective in lowering the cholesterol levels in hypercholesterolemic mice is in concentration P7 (60%) with a reduction of 59.25 mg/dL.

Keywords

noni fruit bay leaf cholesterol combination Balb/c Mice

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1.
Noor A, Nanik Lestariningsih, Lilin Ika Nur Indahsari. The Combination of Bay Leaf (Syzgyum polyanthum) and Noni Fruit (Morinda citrifolia) in Lowering Cholesterol Levels in Balb/C Hypercholesterolemia Mice. EKSAKTA [Internet]. 2025Feb.11 [cited 2025Feb.22];26(01):74-85. Available from: https://eksakta.ppj.unp.ac.id/index.php/eksakta/article/view/557

References

  1. Dinkes Kalteng. (2022). Dinas Kesehatan Prov. Kalteng Gelar Sosialisasi Penggunaan Obat Tradisional Yang Aman Dan Bermanfaat.
  2. Rahmawati, N., Indrian Mustofa, F., Haryanti, S., Subositi, D., Widodo, H., Yanti Marfuatush Sholikhah, I., & Widiyastuti, Y. (2022). Ethnopharmacology Study Of Medicinal Plants Utilized For Hypercholesterolemia Treatment On Borneo Island Of Indonesia. Jurnal Tumbuhan Obat Indonesia, 15(1), 1–15.
  3. Eko, D., Susilo, H., Hertos, M., Arfianto, D. F. (2019). Pada Beberapa Komposisi Media Tanam (Study on Seeding Potency and Seedling of Morinda on the Plant Media Composition).
  4. Erwan, M. O., & Parbuntari, H. (2023). Identifikasi Senyawa Metabolit Sekunder pada Daun Salam (Syzygium polyanthum). 12.
  5. Forcepta, C., & Nisa, K. (2022). Manfaat Buah Mengkudu (Morinda citrifolia) dalam Menurunkan Kadar Kolesterol Total dan LDL pada Penyakit Dislipdemia The Effect of Noni (Morinda citrifolia) Fruit Giving Against Dyslipidemia. Medula, 11.
  6. Phan, T. C. T., Nguyen, T. K. L., Truong, T. P. T., Pham, T. T. N., Huynh, T. G., & Doan, X. D. (2023). Effects of noni fruit extract on the growth performance, digestive enzymes, and stress tolerance of juvenile whiteleg shrimp (Litopenaeus vannamei). Egyptian Journal of Aquatic Research, 49(4), 549–554.
  7. Pratama, D., Suprihadi, A., & Raharjo, B. (2020). Efektivitas Kombinasi Ekstrak Bahan Herbal (Mengkudu, Pepaya, Kunyit) Terhadap Daya Hambat Pertumbuhan Aeromonas hydrophila Secara In Vitro. In Jurnal Biologi, 6(2).
  8. Riyanti, F., & Purwaningrum, W. (2022). The Utilization of Noni Fruit Extract as Antiseptic in the Production of Liquid Soap in Sungai Lacak Village, Pulokerto Sub-District, Gandus District, Palembang City. Sriwijaya Journal of Community Engagement and Innovation, 1(2), 101–109.
  9. Song, J., Fang, Y., Rao, X., Wu, L., Zhang, C., Ying, J., Hua, F., Lin, Y., & Wei, G. (2024). Beyond conventional treatment: ASGR1 Leading the new era of hypercholesterolemia management. In Biomedicine and Pharmacotherapy, 180. Elsevier Masson s.r.l.
  10. Dewi, N. P., Komang, N., Astari, A., & Tuldjanah, M. (2022). Uji Efektivitas Ekstrak Etanol Daun Kluwih Terhadap Gambaran Histopatologi Hati Tikus Putih Hiperkolesterolemia Diabetes. Farmakologika Jurnal Farmasi, 2.
  11. Priadna, A. I., Adiwinoto, B., & Handajani, F. (2019). Pengaruh Ekstrak Buah Mengkudu (Morinda citrifolia) Terhadap Kadar Kolesterol Total Darah Pada Tikus Putih (Rattus norvegicus) Jantan galur Wistar yang Diberi Diet Tinggi Lemak. Medical and Health Science Journal, 3(1), 17–24.
  12. Ariantari, N. P., Yowani, C., Dewa, D., & Swastini, A. (2020). Uji Aktivitas Penurunan Kolesterol Produk Madu Herbal Yang Beredar Di Pasaran Pada Tikus Putih Diet Lemak Tinggi Hypocholesterolemic Activity Of Marketed Herbal Honey Products In Albino Rats With Hypercholesterolemic Diet. Jurnal Kimia, 4(1), 15–19.
  13. Silalahi, M. (2019). Syzygium polyanthum (Wight) Walp. (Botani, Metabolit Sekunder dan Pemanfaatan).
  14. Karimou, R., Salami, H. A., Agossou, E., Boya, B., Assouma, F. F., Bouko, B. O. M. B., Attakpa, E. S., Baba-Moussa, L., & Sina, H. (2024). Assessment of antimicrobial and anti-biofilm activities of lemongrass and bay leaf extracts on microorganisms from fermented cereal-based porridges in northern Benin. Scientific African, 24.
  15. Hijriani, B. I., Atfal, B., Kodariah, L., Hadiatun, N., & Ismatullah, N. K. (2023). Efektivitas Ekstrak Daun Salam (Syzygium polyanthum) Dalam Mencegah Kenaikan Kadar Kolesterol LDL Tikus Putih (Rattus norvegicus) Diinduksi Kuning Telur Puyuh. Jurnal Kesehatan Rajawali, 13(2), 1–4.
  16. Dzah, C. S., Kpodo, F. M. K., Asante-Donyinah, D., & Boateng, N. A. S. (2024). The influence of Morinda citrifolia fruit maturity level, parts and storage length on total phenols, ascorbic acid, antioxidant activity and ethylene gas emission. Food Chemistry Advances, 4.
  17. Sorndech, W., Auranwiwat, C., Ploypetchara, T., & Butseekhot, S. (2024). Enhancing bioactive compounds extraction from noni leaves (Morinda citrifolia) by enzymes-assisted extraction. Journal of Agriculture and Food Research, 18.
  18. Oktaviana, S. (2019). Uji Aktivitas Antibakteri Dari Ekstrak Biji Mengkudu (Morinda citrifolia L.).
  19. Moh, J. H. Z., Waiho, K., Fazhan, H., Shaibani, N., Manan, H., Sung, Y. Y., Ma, H., & Ikhwanuddin, M. (2021). Effect of Noni, Morinda citrifolia fruit extract supplementation on the growth performances and physiological responses of the hepatopancreas of Whiteleg shrimp, Penaeus vannamei Post Larvae. Aquaculture Reports, 21.
  20. Christopher, M., Sutikno, S. D., & Louisa, E. (2021). Artikel Tinjauan Pustaka Tinjauan Pustaka Efek Skopoletin Dari Ekstrak Buah Mengkudu (Morinda citrifolia) Sebagai Modalitas Herbal Dalam Penanganan Preeklamsia. Essence of Scientific Medical Journal, 19(2), 14–19.
  21. Huo, X., Sun, S., Li, K., Zhang, J., Wang, Y., Gao, H., Cao, Z., Xu, X., Ma, G., & Liu, M. (2023). Constituent analysis, laxative activity, and toxicological evaluation of methanol extract of noni fruit (Morinda citrifolia L., Rubiaceae). Food Chemistry: X, 20.
  22. Zhang, C., Gu, C., Su, F., Wang, M., Chen, J., Chang, Z., Zhou, J., Yue, M., Liu, F., & Feng, Z. (2024). Iridoid glycosides from noni (Morinda citrifolia L.) fruit pomace: A novel booster strategy for its extraction and will its α-glucosidase inhibitory be increased by acetylation? LWT, 207.
  23. Prahastuti, S., Tjahjani, S., & Hartini, E. (2021). The Effect Of Bay Leaf Infusion (Syzygium polyanthum (Wight) Walp) To Decrease Blood Total Cholesterol Level In Dyslipidemia Model Wistar Rats. In Jurnal Medika Planta, 1(4).
  24. Ahmad, P. (2020). Pengaruh Pemberian Ekstrak Daun Mengkudu (Morinda citrifolia Linn) Dalam Air Minum Terhadap Plasma Metabolit Burung Puyuh.
  25. Bryda, E. C. (2023). The mighty mouse : The Impact of Rodents on Advances in Biomedical Research. Diakses pada tanggal 14 Januari 2025.
  26. Widiartini, W., Siswati, E., Setiyawati, A., Rohmah, I. M., Prastyo, E. (2019). Pengembangan Usaha Produksi Tikus Putih (Rattus norvegicus) Tersertifikas Dalam Upaya Memenuhi Kebutuhan Hewan Laboratorium.
  27. Mutiarahmi, C. N., Hartady, T., & Lesmana, R. (2021). Use Of Mice As Experimental Animals In Laboratories That Refer To The Principles Of Animal Welfare: A Literature Review. Indonesia Medicus Veterinus, 10(1), 134–145.
  28. Agustin Kusuma Wardani, N., Ina Sarinastiti, D., Tari Indriani. (2020). Penurunan Kadar Kolesterol Total Pada Mencit Jantan Putih Oleh Cincau Kulit Buah Naga Merah Decrease of Total Cholesterol Levels in White Male Mice by Red Dragon Fruit Peel Grass Jelly. Jurnal Pangan dan Agroindustri, 8(2).
  29. Aflikah, S. (2019). Pengaruh Pemberian Kombinasi Ekstrak Kulit Batang Kayu Manis (Cinnamomum Burmanii) Dan Bawang Dayak (Eleutherine Palmifolia (L.) Merr) Terhadap Kadar LDL dan HDL Serum Darah Mencit (Mus musculus) Yang Di Induksi HFD (High Fat Diet).
  30. Lely, N., Rasyad, A. A., & Sundari, A. A. (2022). Uji Aktivitas Antidepresan Fraksi Etil Asetat Ekstrak Daun Sirih Merah (Piper Crocatum Ruiz &Pav.) Terhadap Mencit Putih Jantan Galur Swiss Webster. In Jurnal Ilmiah Bakti Farmasi, 5(2).
  31. Muthmainnah, B. (2019). Skrining Fitokimia Senyawa Metabolit Sekunder Dari Ekstrak Etanol Buah Delima (Punica granatum L.) Dengan Metode Uji Warna. Media Farmasi, 13(2), 36.
  32. Ginaris, P. R. (2020). Lotion Antioksidan Ekstrak Daun Salam (Syzygium polyanthum Wight Walp.). Jurnal Kesehatan Tujuh Belas, 2(1), 182.
  33. Hartanti, L., Maureen Kasih Yonas, S., Jacqlyn Mustamu, J., Wijaya, S., Kurnia Setiawan, H., & Soegianto, L. (2019). Influence of extraction methods of bay leaves (Syzygium polyanthum) on antioxidant and HMG-CoA Reductase inhibitory activity. Heliyon, 5, 1485.
  34. Nazirah, Nasution, M. A., Ridwanto, & Nasution, H. M. (2023). Phytochemical screening and antioxidant activity test of methanol extract salamleaves (Syzygium polyanthum(Wight.) Walp.) from Gampong Bunot Pidie Jaya using the DPPH method. Journal of Pharmaceutical and Sciences, 1(1), 104–116.
  35. Azzahra, R. W., & Zuhrotun, A. (2022). Indonesian Journal of Biological Pharmacy Review Article: Potential Anti-Cholesterol Plants Based on In-Vitro Studies.
  36. Chen, J., Chen, X., Zhang, Y., Feng, Z., Zhu, K., Xu, F., & Gu, C. (2024). Bioactivity and influence on colonic microbiota of polyphenols from noni (Morinda citrifolia L.) fruit under simulated gastrointestinal digestion. Food Chemistry: X, 21.
  37. Anwar, K., & Triyasmono, L. (2020). Kandungan Total Fenolik, Total Flavonoid, dan Aktivitas Antioksidan Ekstrak Etanol Buah Mengkudu (Morinda citrifolia L.). Jurnal Pharmascience, 3(1), 83–92.
  38. Talreja O, Kerndt CC, Cassagnol M. Simvastatin. [Updated 2023 Jun 5]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK532919/
  39. Rusmayadi, G., Waoma, S., Malasari, C. A., Syah, S. P., Sappaile, B. I., & Marpaung, P. (2023). Pelatihan Penggunaan Aplikasi Spss Dalam Pengolahan Data Penelitian. Community Development Journal, 4(1).
  40. Sintia, I., Danil Pasarella, M., & Andi Nohe, D. (2022). Prosiding Seminar Nasional Matematika, Statistika, dan Aplikasinya Terbitan II.
  41. Usmadi. (2020). Pengujian Persyaratan Analisis (Uji Homogenitas dan Uji Normalitas). Inovasi Pendidikan, 7(1), 50–62.