Distribution of blaCTX-M, blaSHV, blaTEM genes in Extended Spectrum Î²-Laktamase Producing Klebsiella pneumoniae from Clinical Isolates in Jakarta

Yulia Engel; Conny Riana Tjampakasari; Fithriyah Sjatha

doi:10.24036/eksakta/vol26-iss02/549

EKSAKTA: Berkala Ilmiah Bidang MIPA

Open Access

Distribution of blaCTX-M, blaSHV, blaTEM genes in Extended Spectrum Î²-Laktamase Producing Klebsiella pneumoniae from Clinical Isolates in Jakarta

Yulia Engel ¹

, Conny Riana Tjampakasari ²

, Fithriyah Sjatha ²

¹ Masterâ€™s Programme in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

² Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

Published 2025-06-30

Vol. 26 No. 02 (2025) PAGES 175-185

DOI 10.24036/eksakta/vol26-iss02/549

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Abstract

Klebsiella pneumoniae is one of the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) due to their high level of antibiotic resistance. Ceftriaxone is one of the cephalosporin antibiotic that functions inhibit bacterial cell wall synthesis and used for treating K. pneumoniae infections. Resistance to ceftriaxone in K. pneumoniae has been widely reported, with one contributing factor being the production of Î²-lactamase enzymes encoded by the genes bla_CTX-M, bla_SHV, and bla_TEM. This study characterized the presence of these genes Â in 12 clinical isolates of Â Â Â Â Â Â Â Â K. pneumoniae and analyzed their correlation with phenotypic resistance to ceftriaxone. All isolates characterized with antimicrobial susceptibility testing (AST) and disk diffusion methods to evaluate the phenotypic production of bla_CTX-M, bla_SHV, and bla_TEM. Molecular analysis using the polymerase chain reaction (PCR) method showed the genes bla_CTX-Mand bla_TEM were detected in 11 isolates (91.67%), and bla_SHV was found in 9 isolates (75%). The distribution pattern of the bla_CTX-M,bla_SHV and bla_TEM resistance genes was present in 8 isolates (66.67%), with MIC values > 64 Âµg/mL. The presence of bla_CTX-M, bla_SHV, and bla_TEM genes together in K. pneumoniae isolates represents a potential risk for resistance to other Î² lactam antibiotics.

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Distribution of blaCTX-M, blaSHV, blaTEM genes in Extended Spectrum Î²-Laktamase Producing Klebsiella pneumoniae from Clinical Isolates in Jakarta

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