Main Article Content

Abstract

Tissue culture is a plant propagation technique using artificial media by adding particular growth regulators (ZPT) to produce plants as expected. Although, the availability of other issues such as browning and contamination can inhibit the process of explant propagation. The addition of cow’s milk to the culture media and BAP is a type of cytokinin is expected to regenerate the growth of banan Kepok Tanjung variety optimally. This study aims to have best concentration of BAP and cow’s milk for the growt Kepok Tanjung explants in vitro. The experimental design used was a randomized with 16 treatment combinations. The result showed a significant of the combination of media treatment of banana Kepok Tanjung variety on the number of leaves (24.2 sheets) and explant height (25.62 cm) starting from 3-12 WAP.

Keywords

Kepok Tanjung, growth, BAP, Musa acuminate balbasiana, cytokinin

Article Details

How to Cite
1.
Rozanah Indira Lestari, Susiyanti, Samsu Hilal, Andi Apriany Fatmawaty. Growth Response of Banana Kepok Tanjung (Musa acuminata balbasiana) with the Application of BAP (Benzyl Amino Purine) and Cow’s Milk In Vitro. EKSAKTA [Internet]. 2024Mar.30 [cited 2024Apr.22];25(01):58-6. Available from: https://eksakta.ppj.unp.ac.id/index.php/eksakta/article/view/473

References

  1. Ayuwira, Milchi. Muslich Hidayat, and Yunizar Hendri. (2021). Effect of Combination of BAP (Benzylamino purine) and TDZ on the Growth of Banana Plant Shoots Kepok Tanjung (Musa acuminata balbisiana) through In Vitro Culture. Journal of Biological Sciences and Applied Biology. Vol.1 (2): 20-27.
  2. Jumjunidang. Riska. Titin Purnama. Agus Sutanto. Resta Patma Yanda. Catur Hermanto. (2021). Resistance of Three Superior New Banana Varieties on Several Vegetative Compatibility Groups Fusarium oxysporum f. sp. cubense. Jurnal Fitopatologi Indonesia. Vol.17 (4):151-158.
  3. Febmita, Elna. Santi Diana Putri. (2023). Test of Several Natural Growth Regulators (ZPT) for Vegetative Propagation of Banana Weevil (Musa paradisiaca L.) Kepok Tanjung Variety. Jurnal Agroplasma. Vol.10 (1):216-226.
  4. Ziraluo, Yan Piter. (2021). Method of Propagation of Purple Sweet Potato Plant (Ipomea batatas pioret) with Tissue Culture Technique or Planlet Cuttings. Jurnal Inovasi Penelitian. Vol.2 (3):1037-1046.
  5. Jannah, K.P.A. I, Prihantoro. Karti. (2023). Optimization of Benzyl Amino Purine (BAP) Levels for the Growth of Butterfly Pea (Clitoria ternatea) Plants through Tissue Culture Techniques. Jurnal Ilmu Nutrisi dan Teknologi Pakan. Vol.21 (2):100-106.
  6. Sulaiman S, Yusuf MA. Awal. A. (2020). Effect of Plant Growth Regulators on In Vitro Culture of Pineapple (Ananas comosis L. Merr) MID2 Variety. Food Research. 4 (4): 110–114.
  7. Saepudin, Adam. Amir. A. Undang. U. Tini. S. (2023). In Vitro Culture of Cavendish Banana (Musa acuminata L.) In Media with Different Concentrations of Stone Guava Extract and Benzyl Amino Purine. Jurnal Ilmiah Pertanian. Vol. 11(1): 87-94.
  8. Nurhanis, Stefani Eka. Wulandari, Reine Suci. Suryanti, Rosa. (2019). Correlation of IAA and BAP Concentration to the Growth of Sengon Tissue Culture. Jurnal Hutan Lestari. Vol.7 (2) : 857-867.
  9. Istianingrum, K. B. dan 1putri, dan 1staf. (2021). Study of the Combination of TDZ and BAP Growth Regulator Treatment on the Development of Red Durian Callus. Jurnal Agrotek Tanaman. Vol.13 (1): 52-60.
  10. Arafah DL, Hernawati D & Nuryadin E. (2021). The Effect Hormone BAP (6-benzyl amino purine) on The Growth of Potato Axilarry Shoots (Solanum tuberosum L.) In Vitro. Jurnal Biologi Tropis. 21 (3): 641-647.
  11. Syamsiah M, Imansyah AA, Suprapti HK & Badriah DS. (2020). Multiplication Response of Moon Orchid (Phalaenopsis sp.) to Addition of Multiple Concentrations of BAP (Benzyl Amino Purine) to In Vitro Media. Agroscience. 10 (2): 148-159.
  12. A.A. Murgayanti. Putri. A, Nuraini. (2021). In Vitro Shoot Multiplication of Curcuma zedoria (Christm.) Roscoe on Various Types of Carbohydrates and Cytokinins. Jurnal Kultivasi. Vol.20 (3): 189-195.
  13. Saepudin, Adam. Yanto, Yulianto. Rida, Nurul Aeni. (2020). Growth of Dendrobium Hybrid Orchid In Vitro Explants on Several Basal Media and Coconut Water Concentrations. Media Penelitian. Vol.5 (2):97-115.
  14. Sigit, Miarsono. Wahyu, Rafida Putri. Junianto .W.A.P. (2021). Comparison of Fat, Protein and Nonfat Dry Matter (BKTL) Content in Fresh Cow's Milk in Kediri City and Kediri Regency. Jurnal Ilmiah Fillia Cendikia. Vol.6 (1): 31-35.
  15. Sri, Lina Brilianty. Suprihatin. Purwoko. (2022). Life Cycle Assessement of Fresh Cow Milk: A Case Study at KPBS Pangalengan. Jurnal Teknologi Industri Pertanian. Vol.32 (3): 220-228.
  16. Krisdianto, Anggi. Endang S. .Yulita .N. Nintya Setiari. (2020). Growth of Phalaenopsis amabilis (L.) Blume Orchid Plantlet On Subculture Stage By Difference of Media Types And Pepton Concentrations. Journal of Biological Sciences. Vol. 7(2) : 182-190.
  17. Samiei, Leila. Mahboubeh, D. Pahnehkolayi. Ali Tehranifar. Zahar Kariman. (2021). Organic and Inorganic Elicitors Enhance In Vitro Regeneration of Rosa Canina. Journal of Genetic Engineering and Biotechnology. 19:60.
  18. Sarah. Endang, Nurcahyani. T. T. Handayani. Mahfut. (2023). Response to Bean Sprout Extract Vigna radiata (L.) R. Wilczek. In Murashige and Skoog Medium Against the Growth of Mustard Green Explants Brassica rapa var. parachinensis L. In Vitro. Jurnal Biologi Makassar. Vol 8(2): 88-95.
  19. Nuraini, A. E. Aprilia. Murgayanti. A.P. Wulandari. (2022). Effect of Benzyl amino purine Concentration On The Growth of Axillary Bud Explants of Ramie Local Clone of Wonosobo in In-Vitro. Jurnal Kultivasi. Vol.21(2): 166-172.
  20. Yanti, Defila. M.N Isda. (2021). Shoots Induction of nodes (Citrus microcarpa Bunge.) with addition 6-Benzyl Amino Purine (BAP) by In Vitro. Journal Biospecies. Vol.14(1): 53-58.
  21. Avivi, Sholeh. M. Ubaidillah. Setiyono. Rifngatul.A. (2022). Effect of BAP, IAA, and Types of Explants on the Regeneration Efficiency of Tomato Fortuna 23. Journal Agron Indonesia. Vol.50(3): 307-314.
  22. Mawaddah, Syerin. K. N.w. Saputro. Ani Lestari. (2021). The Effect of Naphthalene Acetic Acid (NAA) and Kinetin of Shoot Multiplication Ginger Plant (Globba leucantha var. bicolor Holttum) on In Vitro. Bioma. Vol.23(1):43-50
  23. Samudera, Ardika. A. Hadi Rianto. Historiawati. (2019). In Vitro Rooting of Sugarcane Explant (Saccharum officinarum L.) Bululawang Varieties at Various Concentrations of NAA and Sucrose on Sugarcane Planlet Growth. Jurnal Ilmu Pertanian Tropika dan Subtropika. Vol.4(1): 5-13.
  24. Desy. Mukarlina. Elvi. R. (2023). Growth of meristem Culture's Crown from Ananas comosus (L. Merr.) In Murashige Skoog (MS) Media with Addition of Corn (Zea mays (L.) and Naphthalene Acetic Acid (NAA) Extract. Jurnal Biologica Samudra. Vol. 5(1): 28-40.
  25. Karamina, H. E, Indawan. F.I.K. Agustina. (2022). Effectiveness of Differences BAP Concentrations on The Growth of Cavendish Banana Planlets with Thin Cells Layer Technique. Jurnal Kultivasi. Vol. 21(2): 135-140.
  26. Saputri, Mulia. Marai Rahmawati. Elly Kesumawati. (2019). Accretion of Barangan Banana Shoot Effect of BAP and Activated Charcoal Explant by In Vitro. Jurnal Ilmiah Mahasiswa Pertanian. Vol.4(1): 73-91.
  27. Sakina, Siti. Syaiful Anwar. F. Kusumawati. (2019). In Vitro Dendrobium Orchid (Dendrobium sp.) Plantlet Growth in Different Concentration of BAP and NAA. Jurnal Pertanian Tropik. Vol.6(3): 430-437.
  28. Khatri, Poonam. J.S. Rana. Anil Sindhu. Pragati Jamdagni. (2019). Effect of additives on enhanced in-vitro shoot multiplication and their functional group identification of Chlorophytum borivilianum Sant. Et Fernand. SN Applied Sciences. Vol. 1(1):1-10.
  29. Astutik. Sutoyo. Astri Sumiati. (2021). Use of Alar and Benzyl Adenine (BA) in Banana Meristem Multiplication. Jurnal Buana Sains. Vol 21(2):7-14.
  30. Bapat, Vishwas. A. Umesh. B. Jagttap. Siddhesh B. Ghag. T.R. Ganapathi. (2020). Molecular Approaches for the Improvement of Under-Researched Tropical Fruit Trees: Jackfruit, Guava, and Custard Apple. International Journal of Fruit Science. Vol.20(3): 233-281.
  31. Karyaningytyas, A.W. Ani Lestari. Edhi, Sandra. (2023). Effect of Several Cytokinin Formulations on Seed Supply and Growth of Pandak Pule Plant Explants (Rauvolfia serpentina (L.) Benth. ex Kurz) In Vitro. Jurnal Agroplasma. Vol.10(1): 237-251.