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Abstract

The Study about Analysis of Nepenthes Hybrids in Taratak Hill, Pesisir Selatan Sumatera Barat by RAPD Technique had been conducted from May until November 2011. The study was done to prove natural hybrids of Nepenthes by RAPD technique and to determine the relationship of natural hybrids of Nepenthes with both parental. The research method is survey and observation. Isolation of DNA carried by CTAB method with modifications and amplification carried out by PCR. Hybrids and parental relationship was obtained from Jaccard similarity coefficient and displayed in a Dendogram (family tree) and PCO analysis to look genetic variation was processed with the program PAST 2.10. Proof of the natural hybrid N. ampullaria × N. mirabilis can be done by the RAPD technique using the primers OPA 15, OPK 16, OPK 19 and OPP 15. Natural hybrids between N. ampullaria × N. gracilis using the primers OPA 15, OPK 19 and OPP 15. The eighth of natural hybrids N. ampullaria × N. mirabilis were closer to N. mirabilis, while both natural hybrids N. ampullaria × N. gracilis closer to the N. Ampullaria.

Keywords

Nepenthes RAPD Natural Hybrid

Article Details

How to Cite
1.
Enjelina W, Mansyurdin M, Meideliza T. Analysis of Nepenthes Hybrids in Bukik Taratak West Sumatra by RAPD Technique. EKSAKTA [Internet]. 30Oct.2018 [cited 24Feb.2020];19(2):12-0. Available from: http://eksakta.ppj.unp.ac.id/index.php/eksakta/article/view/137

References

  1. [1] Bittleston LS, Wolock CJ, Yahya BE, Chan XY, Chan KG, et al. 2018. Convergence between the microcosms of Southeast Asian and North American pitcher plants. eLife 7
  2. [2] Lim RJY, Lam WN, Tan HTW. 2018. Novel pitcher plant-spider mutualism is dependent upon environmental resource abundance. Oecologia 188:791-800
  3. [3] Gogoi B, Bhau BS. 2018. DNA barcoding of the genus Nepenthes (Pitcher plant): a preliminary assessment towards its identification. BMC plant biology 18:153
  4. [4] Miguel S, Hehn A, Bourgaud F. 2018. Nepenthes: State of the art of an inspiring plant for biotechnologists. Journal of biotechnology 265:109-15
  5. [5] Wan Zakaria WN, Loke KK, Zulkapli MM, Mohd Salleh F, Goh HH, Mohd Noor N. 2015. RNA-seq Analysis of Nepenthes ampullaria. Frontiers in plant science 6:1229
  6. [6] Chou LY, Dykes GA, Wilson RF, Clarke CM. 2016. Nepenthes ampullaria (Nepenthaceae) Pitchers Are Unattractive to Gravid Aedes aegypti and Aedes albopictus (Diptera: Culicidae). Environmental entomology 45:201-6
  7. [7] Sri Rahayu 2010 Kajian Fertilitas Hibrid Alami pada Populasi Kantung Semar (Nepenthes) di Bukit Taratak, Kanagarian Surantih, Kecamatan Sutera, Kabupaten Pesisir Selatan Skripsi Sarjana Biologi FMIPA, Universitas Andalas
  8. [8] Muhammadin 1995 Studi Taksonomi Nepenthes yang terdapat di Bukit Taratak, Pesisir Selatan, Skripsi Sarjana Biologi FMIPA, Universitas Andalas.
  9. [9] Brito, L., A. Carvalho, A. Martin, J.S.H. Harrison and G. Pinto. 2006. Morphological, yield, cytological and molecular characterization of a bread wheat × tritordeum F1 hybrid. Indian Academy of Sciences 85(2): 123-131
  10. [10] Dhillon,R.S, M.S.Hooda, M.Jattan, V.Chawla. M.Bhardwaj and S.C. Goyal. 2009. Development and Molecular Characterization of Interspesific Hybrid of Jatropha curcas x J. integerrima. Indian Journal of Biotechnology. 8:384-390
  11. [11] Trijatmiko, K. R., 2006, Penggunaan Metode PCR untuk Deteksi Cepat Keragaman DNA. Pelatihan dan Workshop Identifikasi DNA dengan Aplikasi PCR. Balai Penelitian Tanaman Jeruk dan Buah Subtropika-BPPT. Sumatera Barat
  12. [12] Zhang, H.Y, F.S. Li, l. L. He, H.Q.Zhong, Q.H.Yang and S. C. He. 2008. Identification of sugarcane interspecies hybrids with RAPDs. African Journal of Biotechnology.7(8): 1072-1074
  13. [13] Akhriadi. 2007. Kajian Taksonomi Hibrid Alami Nepenthes Di Kerinci. Thesis Sarjana Biologi FMIPA, Universitas Andalas
  14. [14] Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18(22): 6531-6535
  15. [15] Mehetre, S.S., A.R. Aher, G.C. Shinde, M. Gomes and S. Eapen. 2004. RAPD analysis of interspesific hybrid between Gossypium arboreum and Gossypium stocksii. Caryologia 57(2): 167-171
  16. [16] Anuniwat, A., A. Chaveerach, T. Tanee and R. Sudmoon. 2009. Development of SCAR Markers for Species Identification of the Genus Nepenthes (Nepenthaceae). Pak. J. Biol. Sci. 12 (22): 1455-1461[11] Zangari L-K T a G 2014 Modification of TiO2 Nanotubes by Cu2O for Photoelectrochemical, Photocatalytic, and Photovoltaic Devices, Electrochimica Acta 128 341-348
  17. [17] Bhau, B.S., K. Medhi, T. Sarkar, S.P. Saikia. 2009. PCR based molecular characterization of Nepentheskhasiana Hook. f. –pitcher plant. Genet Resour Crop Evol. 56: 1183 – 1193
  18. [18] Smithies, O. 1958. An Improved Procedure for Starch-Gel Electrophoresis Further Variations in the Serum Protein of Normal Individual. Biochem. 21: 585-587
  19. [19] Akhiardi P., Hernawati and R. Tamin. 2007. The natural hybrids of Nepenthes in Sumatra. Abstract : Serawak Nepenthes Summit, 18 – 21 August 2007. Serawak Forestry, Malaysia

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