Biomol Potential from Vegetable Market and Tofu Factory Waste for Cleared Land Crops

. This research was conducted in Cialam Caya Village, Konda Subdistrict, South Konawe Regency, in July - September 2021. The purpose of the study was to determine the effect of various doses of Biomol on the growth and yield of mustard greens. This study was arranged in a Randomized Block Design (RAK) which consisted of 7 treatments with biomol doses, namely: control (without biomol), P1 50 ml, P2 100 ml, P3 150 ml, P4 200 ml, P5 250 ml, P6 300 ml, 3-time treatment repetition to produce 21 experimental units. The results of this study concluded that the administration of biomol had a very significant effect on the growth and yield of mustard greens (plant height, number of leaves, fresh weight and dry weight) and the best dose of biomol for mustard greens ( Brassica Juncea L.) was at a dose of 200 ml. This


Introduction
The traditional market in Cialam Caya Village, Konda Subdistrict, is the largest in Southeast Sulawesi Province's South Konawe Regency. The Cialam Caya Village Market of Konda Subdistrict is a center for vegetable commodities and a gathering spot for traveling traders to fill their wares due to the habits of people who want to eat vegetables. Cabbage and mustard greens are the most extensively sold veggies, according to preliminary findings at the Cialam Caya Village Market of Konda Subdistrict. Many cabbage and mustard greens are not sold as a result of this process, and they may end up as vegetable market waste. Market waste, such as mustard greens and cabbage, is the vegetables that are no longer fit for sale or eating due to deterioration. According to Kurniawan (2018) [1], the disadvantage of cabbage and mustard green waste is its high water content (over 90%), which causes it to deteriorate quickly and necessitates its sale or processing right away. In addition, there is a tofu factory in the Cialam Caya Village of Konda Subdistrict that produces a lot of organic waste.
Organic waste in the form of vegetable and tofu factory waste has begun to garner attention, according to the facts on the ground [17]. Every day, more waste is produced, making it increasingly difficult to find a disposal location. The traders in the market's corner just collect the waste of cabbage and mustard greens. The garbage officer will carry the gathered material to the final disposal site. In the meantime, tofu factory waste is occasionally used as animal feed. Given the vast volume of lost vegetable and tofu factory waste, this vegetable and tofu factory waste has the potential to be exploited. In the Cialam Caya Village Market of Konda Subdistrict, the utilization of vegetable and tofu factory waste is currently not being used properly. In the discipline of microbiology, the waste problem can be solved by fermenting it for at least 5 days.
Biomol is an extract made from the fermented product of vegetable market cabbage and mustard green trash [18,19,20,21,22]. Biomol is made by crushing vegetable waste, sprinkling it with a lot of salt, and then storing it in a tightly sealed container for several days. The solution is then filtered and ready to use as an organic liquid fertilizer [2]. The use of a liquid fertilizer containing biomol extract helps to meet the soil's nutritional requirements [23,24,25,26,27]. Because it contains microorganisms that break down organic debris, encourage development, and act as a biological controller, Biomol solution is suitable for use as an organic pesticide [3]. Several researchers, including (Sasmita et al., 2014) [4], have conducted biomol research, with the conclusion that administering local microorganisms (MOL) from the fruit promotes asexual growth in sweet potato plants. According to the findings of (Rahmah et al., 2014) [5], liquid organic fertilizers of varied concentrations had an effect on corn development. The combining of vegetable and tofu factory waste into organic fertilizer distinguishes this study from the two others, that will be conducted by the researcher.
In fact, many individuals in Konda Subdistrict's Cialam Caya Village utilize chemical fertilizers instead of organic fertilizers for their cleared land's food crops. Chemical fertilizers have increased nutrient elements that are easier to obtain, even at high prices. Continuous use of chemical fertilizers results in a loss of key nutrients in the soil and a deterioration in fertility. As a result, in addition to being uneconomical for food crops on the house's cleared land, it also pollutes the soil and water environment. As a result, organic fertilizers that are both non-toxic to plants and readily available in the environment are required, one of which is biomol, which is made up primarily of vegetable and tofu factory waste.
On general, the community around the Cialam Caya traditional market of Konda Subdistrict has used vegetable waste for food plants in their cleared land of the house, but simply put it on the ground around food plants in the hopes of degrading into compost. Kale, spinach, and fruits are just a few of the many food crops that the inhabitants of Cialam Caya Village, Konda Subdistrict, raise on their cleared land. Because people restrain themselves to going to the market, the food plants in this house's cleared land appear to be valuable during the Covid-19 outbreak.
Processing vegetable market and tofu factory waste into biomol is efficient, simple, inexpensive, and environmentally friendly, and it is projected to boost food crop yields during the Covid-19 pandemic. Aside from these considerations, it is important to undertake a study titled "Biomol Potential from Vegetable Market and Tofu Factory Waste for Cleared Land Corps".
Tools and Materials: (1) Making of Biomol, Biomol solution was made by mixing a solution of sugar and EM-4 into water with a ratio of 50 ml of sugar solution: 50 ml of EM-4: 1 liter of water, then let stand for one day. Organic waste (vegetable residue) taken from the market was cut into small pieces of approximately 1-2 cm, then dried, then mixed with tofu factory waste (2:1:1). Furthermore, the material was put into a tightly closed tub. The temperature was not more than 40°C. Biomol will be ready for use after seven days of fermentation, according to Ariska et al. (2012) [6]; (2) preparation of verticulture shelves, the verticulture shelves were formed of gutters stacked in layers with wooden supports with a model like steps. The gutter is 900 cm in length, 10 cm in width, and 10 cm in height. The gutters were then stacked vertically like a ladder on the wood, with up to 5 gutters per vertical shelf and a 30 cm gap between gutters. Each level of the gutter was used to create a duplicate.

Figure 2. Observations of Data Collection
The purpose of data analysis was to assess the influence of treatment on the observed variables, and then F (ANOVA) was used to analyze each observation. If there was a real effect, a follow-up test with a 5% Honestly Significant Difference (HSD) would be conducted. A Randomized Block Design Variety Print Analysis was used to statistically analyse observational data from each treatment.

Plant Height
Analysis of variance showed that the administration of Biomol at observations 2-5 MST had a very significant effect on the average plant height. The average plant height of 2 MST -5 WAP is presented in Table 1. The results of the 5% HSD test in Table 1 show that the Biomol treatment with a dose of 200 ml (P4) obtained higher plants, but at 3 MST, which was not different from the 150 ml (P3), 250 ml (P5), and 300 ml (P6) treatments. At 4 MST observation, it was different from 100 ml (P1), 150 ml (P2), 250 ml (P5), and 300 ml (P6), and at 5 MST observation, it was not different from 100 ml (P2), 150 ml (P3), and 250 ml (P5) while the lowest plant height was in the control treatment (P0).
The Biomol dosage treatment had a very substantial effect on plant height at 2-5 MST, according to the results of the analysis of variance. Biomol produced the best plant growth results at a dose of 200 ml when compared to the other doses. Because the nitrogen included in Biomol is the most important nutrient, plants' needs for nitrogen are larger than other nutrients, a dose of 200 ml Biomol is capable of supplying nitrogen in accordance with the amount required for growth and development in mustard green. According to Erawan et al., element N is involved in plant vegetative growth, nitrogen is an important ingredient for cell division and elongation, and so N is a component of protoplasm. According to Erawan et al. (2013) [7], element N plays a role in plant vegetative growth, nitrogen is a necessary component for cell division and elongation, and so N is a constituent of protoplasm, which is widely distributed in tissues such as growing points.
The decrease in height development of mustard green after P4 treatment was suspected because the fertilizer dose was not balanced or exceeded the quantity required by the plant after P4 treatment (200 ml of liquid Biomol). According to Nissa (2016) [8], correct and balanced nutrition is required since nutritional deficits or excess nutrients can promote non-optimal plant growth. Excess phosphorus inhibits plant growth owing to N-P bonds that make it harder for plants to absorb nitrogen, while excess calcium alters soil pH, causing plants to become stunted, leaves to turn yellow, and plants to be low, according to [9].
The P3 treatment had the highest average value, indicating that the macro and micronutrient content of the liquid Biomol was enough for the caisim mustard green's needs. Growing plants, according to Parintak (2018) [10], will offer optimal productivity if sufficient nutrients are supplied. According to Gunawan et al., (2011) [11], the leftovers of fruits and vegetables include 23 mg of calcium and 12 mg of phosphorus, which aid in the growth of caisim mustard green height.
The variation in mustard green height at each concentration of the solution was owing to the fact that liquid Biomol includes nutrients that promote mustard green height growth. Plants can consume liquid Biomol with a concentration of 200 ml/L of water in the form of macro and micro nutrients that enter through the leaf mouth (stomata) during the vegetative growth period, resulting in the best plant height when compared to the concentration above it.

Number of Leaves
Number of Leaves. The results of the analysis of variance showed that 2-5 MST observations had a very significant effect. The average number of leaves of plants of 2-5 MST is presented in Table 2. Remarks: The average value followed by the same letter in the same column, not significantly different at the 5% HSD test level Based on the results of the 5% HSD test in Table 4.2, it is showed that the Biomol treatment at a dose of 200 ml (P4) gave the highest number of leaves compared to other treatments, but at 2 MST, it was not different from 100 ml (P2), 150 ml (P3) 250 ml (P5), and 300 ml (P6). The lowest number of leaves was found in the control treatment P0 (without Biomol).
The Biomol dosage treatment had a very substantial effect on the number of leaves at 2-5 MST, according to the analysis of variance. Biomol produced the most leaves at a dose of 200 ml when compared to the other doses. This demonstrates that a dose of 200 ml Biomol is capable of supplying nitrogen in the amount required for the growth and development of mustard greens, as nitrogen nutrients play a critical role in plant vegetative growth, such as plant height and leaf number. Treatment P4 had a higher average growth rate of plant leaves than treatments P0 to P3, according to statistics. Treatment doses of vegetable and tofu factory waste ranging from 50 ml to 200 ml Biomol are thought to be able to satisfy the ideal conditions for plant nutritional needs. Meanwhile, the P5 treatment dose (250 mL liquid Biomol) was thought to be more than the amount of nutrients required by mustard greens. According to Firdaus et al., (2014) [12], if the nutrients provided surpass the amount that the plants require, the increase in the number of plant leaves will not occur ideally. Excess nitrogen fertilizers can induce plant poisoning by lowering soil pH and binding other nutrients, making it difficult for plants to absorb them.
The P3 treatment (250 mL liquid Biomol) produced the highest average value for the number of leaves produced by the caisim mustard green. When compared to other treatments, the P3 treatment (250ml liquid Biomol) contained nitrogen (N) and phosphorus (P) nutrients in accordance with the needs of mustard greens. In line with Indrajaya and Suhartini (2018) [13] assertion that if plants are provided enough nitrogen, their need for other nutrients such as phosphorus increases to compensate for their rapid growth rate. The elements in liquid Biomol are not in excess or deficiency, allowing the mustard greens to thrive to their full potential. A rise in the number of leaves on caisim mustard greens can also be linked to the height of the plant. When a plant grows taller, the number of growth tips on its leaves increases, resulting in more leaves.

Fresh Weight
The results of the analysis of variance showed that the treatment tested had a significant effect on the fresh weight of the mustard greens. The average plant fresh weight for each treatment is presented in Table 3. Remarks: The average value followed by the same letter in the same column, not significantly different at the 5% HSD test level Based on the results of the 5% HSD test in Table 3, it is shown that the 200 ml dose of Biomol (P4) gave a higher fresh weight than the other treatments, while the lowest fresh weight was in the control treatment P0, but not significantly different from the 150 ml treatment (P3 ).

ISSN : 1411 3724 Eksakta : Berkala Ilmiah Bidang MIPA
The Biomol dose treatment had a highly significant effect on the average fresh weight, according to the analysis of variance. When compared to the other doses, the 200 ml Biomol dose produced the highest average fresh weight. This is because the leaves have the largest water and nutrient content, resulting in the maximum plant fresh weight. According to Zulputra and Hidayat (2018) [14], plants still require a lot of energy and nutrients to achieve optimal plant fresh weight, so increasing the number and size of cells can achieve optimal results and allow an increase in the optimal water content of plants as well, as water content accounts for the majority of plant fresh weight. Because water is essential for cell turgidity, the leaf cells will grow.
The addition of biomol to the soil can increase soil porosity, preventing the soil from becoming too dense, resulting in improved root respiration. As a result, root development is more extensive in order to obtain nutrients (both macro and micronutrients) from the soil, and vegetative growth is improved. The heavier the biomass weighed on the plant crown, the better the vegetative development. It's thought that the nutrients in biomol are actually used by plants to raise plant weight at the optimal level. The concentration of biomol with a high content of one macro element can be used by plants to develop the vegetative part of the plant for the better at the optimum point, allowing the metabolic process to operate more smoothly. This is in line with Rinanto et al., (2015) [15], who claim that plants require macronutrients to boost photosynthesis and activate enzymes involved in the process.

Dry Weight
The results of the analysis of variance showed that the treatment tested had a very significant effect on the average dry weight. The average dry weight of plants in each treatment is presented in Table  4.